Inhibition of APC-mediated apoptosis of activated T lymphocytes

Details Inhibition of APC-mediated apoptosis of activated T lymphocytes Inhibition of APC-mediated apoptosis of activated T lymphocytes

1995-02-27

1998-12-01

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

436506, 530387, C12Q 170, G01N 33564, C07K 1600

Patent

active

058436359

ABSTRACT:
Methods for interfering with antigen presenting cell-mediated priming of resting peripheral blood T lymphocytes to undergo activation induced cell death ("apoptosis") by inhibiting an interaction between a membrane associated molecule ("an APC apoptotic ligand") present on stimulated antigen presenting cells ("APC") and a counter-receptor that is present on T lymphocytes are disclosed. The antigen presenting cells are preferably from the monocyte/macrophage cell line or are dendritic cells. Also disclosed are methods of screening for inhibitors of APC-mediated priming of T lymphocytes to undergo apoptosis and methods and agents for detecting, identifying and characterizing an APC apoptotic ligand. Inhibitors identified by the screening method of the invention are used to reduce the T lymphocyte depletion associated with HIV infection and thereby mitigate the severe immunodeficiency associated with AIDS by interfering with the association between HIV-infected antigen presenting cells, especially monocytes and macrophages, and T cells.

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