Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-05-12
1998-06-09
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435361, G01N 3358, G01N 33554, C07K 14655
Patent
active
057632006
ABSTRACT:
A method of determining the ability of a compound to both bind to somatostatin type-5 receptor ("SSTR-5") and inhibit amylin release. The method includes obtaining a preparation, either a cell preparation or a membrane preparation, which contains SSTR-5; incubating the preparation, the compound, and a SSTR-5 ligand, at least one of the ligand and the compound being detectably labeled; determining the ability of the compound to compete against the ligand for binding to SSTR-5; if and only if the compound is determined to be able to bind to SSTR-5, obtaining pancreatic cells; incubating the compound, the pancreatic cells, and an amylin release stimulator under conditions in which the amylin release stimulator would induce release of amylin from the pancreatic cells; and determining the ability of the compound to inhibit amylin release. Also disclosed is a method of treating hyper-amylinemia with a ligand selective for SSTR-5.
REFERENCES:
Davenport et al., "Somatostatin Type 5 Receptors Mediate the Inhibitory Action of Somatostatin on Insulin Secretion in Zucker Fatty Rat Perfused Pancreas", Diabetologia (Supp. 1) 38:A106, XP000602793, 1995.
Moldovan et al., "Somatostatin Inhibits B-Cell Secretion via a Subtype-2 Somatostatin Receptor in the Isolated Perfused Human Pancreas", J. of Surgical Research 59:85-90, XP000602464, 1995.
Hoyer et al., "Molecular Pharmacology of Somatostatin Receptors", Naunyn-Schmiedeberg's Arch Pharmacology 350:441-453, 1994.
Inoue et al., "Effects of Exogenous Somatostatin and Insulin on Islet Amyloid Polypeptide (Amylin) Release from Perfused Rat Pancreas", Horm. Metab. Res. 24:251-253, 1992.
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Cawthorne Michael Anthony
Davenport Michelle
Dunmore Simon Jon
Biomeasure Incorporated
Conway John D.
Hayes Robert C.
Hutzell Paula K.
McGowan William
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