Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1995-06-02
1998-06-09
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
A61K 3800, C07K 500, C07K 700, C07K 1600
Patent
active
057635714
ABSTRACT:
Disclosed are Ras-binding peptides and peptide mimetics thereof which inhibit the direct binding of Ras with Raf and contain an amino acid sequence with 80-100% identity to the sequence of specific Raf fragments.
REFERENCES:
Beardsley, T., "Design for Living: A Signaling Pathway Found in Many Species is Mapped", 1994,Scientific American, pp. 28 and 32.
Chung, D.L., et al., "A Peptide from the Gap-Binding Domain of the ras-p21 Protein as well as Azatyrosine Block ras-Induced Maturation of Xenopus Oocytes", 1991, Biochem. and Biophys. Research Commun., 181(3):1378-84.
Chung, D.L., et al., "A Peptide from the GAP-Binding Domain of the ras-p21 Protein and Azatyrosine Block ras-Induced Maturation of Xenopus Oocytes", 1991, Anticancer Research, 11:1373-78.
Cook, S.J., et al., "Inhibition by cAMP of Ras-Dependent Activation of Raf", 1993, Science 262:1069-72.
Crews, C.M., et al., "Extracellular Signals and Reversible Protein Phosphorylation: What to Mek of It All", 1993, Cell: Minireview, 74(2), pp. 215-217.
Feig, L.A., "The Many Roads That Lead to Ras", 1993, Science, 260:767-68.
Hall, A., "A Biochemical Function for Ras-At Last", 1994, Science, 264:1413-14.
Koide, H., et al., "GTP-dependent Association of Raf-1 with Ha-Ras: Identification of Raf as a Target Downstream of Ras in Mammalian Cells", 1993, Proc. Natl. Acad. Sci. USA, 90(18):8683-86.
Leevers, S.J., et al., "Requirement for Ras in Raf Activation is Overcome by Targeting Raf to the Plasma Membrane", 1994, Nature, 369:411-14.
Luban, J., et al., "Genetic Assay for Multimerization of Retroviral gag Polyproteins", 1992, J. Virology, 66(8):5157-60.
Moodie, S.A., et al., "Complexes of Ras-GTP with Raf-1 and Mitogen-Activated Protein Kinase Kinase", 1993, Science, 260:1658-61.
Mulcahy, L.S., et al., "Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 Cells", 1985, Nature, 313:241-43.
Perrimon, N., et al., "The Torso Receptor Protein-Tyrosine Kinase Signaling Pathway: An Endless Story", 1993, Cell, 74(2):219-22.
Schaber, M.D., et al., "Ras Interaction with the GTPase-Activating Protein (GAP)", 1989, Proteins: Structure, Function, and Genetics, 6:306-15.
Scolnick, E.M., "The Partnership of Academia and Industry in Pharmacologic Research", 1991, J. Lab. Clin. Med., 117(1):8-14.
Van Aelst, L., et al., "Complex Formation Between RAS and RAF and Other Protein Kinases", 1993, Proc. Natl. Acad. Sci. USA, 90:6213-17.
Vojtek, A.B., et al., "Mammalian Ras Interacts Directly with the Serine/Threonine Kinase Raf", 1993, Cell, 74(1):205-14.
Warne, P.H., et al., "Direct Interaction of Ras and the Amino-Terminal Region of Raf-1 In Vitro", 1993, Nature, 364:352-55.
Wu, J., et al., "Inhibition of the EGF-Activated MAP Kinase Signaling Pathway by Adenosine 3',5'-Monophosphate", 1993, Science, 262:1065-69.
Zhang, X., et al., "Normal and Oncogenic p21.sup.ras Proteins Bind to the Amino-Terminal Regulatory Domain of c-Raf-1", 1993, Nature, 364:308-13.
International Search Report, PCT/US94/06654, filed 10 Jun. 1994 (Search Report mailed Aug. 29, 1994).
Bonner et al.; The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene; Nucleic Acids Research; 14:1009-1015 ((1986).
Bruder et al.; Serum-, TPA-, and Ras-induced expression from Ap-1/Ets-driven promoters requires Raf-1 kinase; Genes & Development; 6:545-556 (1992).
Chien et al.; The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest; Proc. Natl. Acad. Sci. USA; 88:9578-9582 (1991).
Durfee et al.; The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit; Genes & Development; 7:555-569 (1993).
Guan et al.; Eukaryotic Proteins Expressed in Escherichia coli: An Improved Thrombin Cleavage and Purification Procedure of Fusion Proteins with Glutathione S-Transferase; Analytical Biochemistry; 192:262-267.
Heidecker et al.; The Role Of Raf-1 Phosphorylation in Signal Transduction; Viral Pathology Section; Laboratory of Viral Carcinogenesis; NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702.
Howe et al.; Activation of the MAP Kinase Pathway by the Protein Kinase raf; Cell; 71:335-342 (1992).
Huleihel et al.; Characterization of Murine A-raf, a New Oncogene Related to the v-raf Oncogene; Molecular and Cellular Biology; 6:2655-2662 (1986).
Itoh et al.; The Post-translational Processing of rasp21 Is Critical for Its Stimulation of Mitogen-activated Protein Kinase; The Journal of Biological Chemistry; 268:3025-3028 (1993).
Kitayama et al.; Genetic analysis of the Kirsten-ras-revertant 1 gene: Potentiation of its tumor suppressor activity by specific point mutations; Proc. Natl. Acad. Sci. USA; 87;4284-4288 (1990).
Kolch et al.; Raf-1 protein kinase is required for growth of induced NIH/3T3 cells; Nature; 349:426-428 (1991).
Kovacina et al.; Insulin Activates teh Kinase Activity of the Raf-1 Proto-oncogene by Increasing Its Serine Phosphorylation; The Journal of Biological Chemistry; 265:12115-12118 (1990).
Kyriakis et al.; Raf-1 activates MAP kinase-kinase; Nature; 358:417-421 (1992).
Marshall et al.; Characterization of Ras effector mutant interactions with the NF1-GAP related domain; Oncogene; 8:425-431 (1993).
Marshall et al.; Identification of Amino Acid Residues Required for Ras p21 Target Activation; Molecular and Cellular Biology; 11:3997-4004 (1991).
Mizuno et al.; A stimulatory GDP/GTP exchange protein for smg p21 is active on the post-translationally processed form of c-Ki-ras p21 and rhoA p21; Proc. Nat. Acad. Sci. USA; 88:6442-6446 (1991).
Quilliam et al.; Biochemical Characterization of Baculovirus-Expressed rap1A/Krev-1 and Its Regulation by GTPase-Activating Proteins; Molecular and Cellular Biology; 10:2901-2908 (1990).
Satoh et al.; Function of Ras as a Molecular Switch in Signal Transduction; The Journal of Biological Chemistry; 267:24149-24152 (1992).
Stanton et al.; Definition of the Human raf Amino-Terminal Regulatory Region by Deletion Mutagenesis; Molecular and Cellular Biology; 9:639-647 (1989).
Tsuda et al.; A Protein Kinase Similar to MAP Kinase Activator Acts Downstream of the Raf Kinase in Drosophila; Cell; 72:407-414 (1993).
Valencia et al.; The ras Protein Family: Evolutionary Tree and Role of Conserved Amino Acids; Biochemistry; 30:4637-4647 (1991).
Avruch Joseph
Marshall Mark S.
Zhang Xian-feng
Harle Jennifer
Indiana University Foundation
The General Hospital Corporation
Tsang Cecilia J.
LandOfFree
Inhibiting protein interactions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Inhibiting protein interactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inhibiting protein interactions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2200475