Inhibiting protein interactions

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

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514 13, 514 14, 514 15, A61K 3800

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active

061036927

ABSTRACT:
The invention discloses methods of inhibiting direct binding of Ras with Raf and screening methods to identify compounds which inhibit direct binding of Ras to Raf, Raf activation, and cell proliferation.

REFERENCES:
patent: 5582995 (1996-12-01), Avruch et al.
patent: 5597719 (1997-01-01), Freed et al.
Andersson et al., "Cloning, Structure, and Expression of the Mitochondrial Cytochrome P-450 Sterol 26-Hydro-xylase, a Bile Acid Biosynthetic Enzyme", J. Biol. Chem., 264:8222-29 (1989).
Avruch et al., "Raf meets Ras: Completing the Framework of a Signal Transduction Pathway", TIBS, 19:279-83 (1994).
Barnard et al., "Identification of the Sites of Interaction Between c-Raf-1 and Ras-GTP", Oncogene, 10:1283-90 (1995).
Bodor et al., "A Strategy for Delivering Peptides into the Central Nervous System by Sequential Metabolism", Science, 257:1698-1700 (1992).
Brtva et al., "Two Distinct Raf Domains Mediate Interaction with Ras", J. Biol. Chem., 270:9809-12 (1995).
Cadwallader et al., "N-Terminally Myristoylated Ras Proteins Require Palmitoylation or a Polybasic Domain for Plasma Membrane Localization", Mol. and Cell. Biol., 14(7):4722-30 (1994).
Chow et al., "Functional Mapping of the N-terminal Regulatory Domain in the Human Raf-1 Protein Kinase", J. Biol. Chem., 270:14100-106 (1995).
Chuang et al., "Critical Binding and Regulatory Interactions Between Ras and Raf Occur Through a Small, Stable N-terminal Domain of Raf and Specific Ras Effector Residues", Mol. Cell. Biol., 14:5318-25 (1994).
Friden et al., "Blood-Brain Barrier Penetration and in Vivo Activity of an NGF Conjugate", Science, 259:343-77 (1993).
Fridman et al., "The Minimal Fragments of c-Raf-1 and NF1 That Can Suppress v-Ha-Ras-induced Malignant Phenotype", J. Biol. Chem., 269:30105-108 (1994).
Ghosh et al., "Identification of Discrete Segments of Human Raf-1 Kinase Critical for High Affinity Binding to Ha-Ras", J. Biol. Chem., 269:30785-88 (1994).
Ghosh et al., "The Cysteine-rich Region of Raf-1 Kinase Contains Zinc, Translocates to Liposomes, and is Adjacent to a Segment that Binds GTP-Ras", J. Biol. Chem., 269:10000-7 (1994).
Guan et al., "Eukaryotic Proteins Expressin in Escherichia coli: An Improved Thrombin Cleavage and Purification Procedure of Fusion Proteins with Glutathione S-Transferase", Anal. Biochem., 192:262-76 (1991).
Hafner et al., "Mechanism of Inhibition of Raf-1 by Protein Kinase A", Mol. and Cell Biol., 14:6696-703 (1994).
Herrmann et al., "Quantitative Analysis of the Complex Between p21.sup.ras and the Ras-binding Domain of the Human Raf-1 Protein Kinase", J. Biol. Chem., 270:2901-905 (1995).
Hu et al., "Cysteine-rich Region of Raf-1 Interacts with Activator Domain of Post-translationally Modified Ha-Ras", J. Biol. Chem., 270(51):30274-77 (1995).
Kyriakis et al., "Mitogen Regulation of c-Raf-1 Protein Kinase Activity Toward Mitogen-activated Protein Kinase-kinase", J. Biol. Chem., 268:16009-19 (1993).
Leevers et al., "Requirement for Ras in Raf Activation is Overcome by Targeting Raf to the Plasma Membrane", Nature, 369:411-14 (1994).
Luo et al., "Identification of the 14.3.3 .zeta. Domains Important for Self-association and Raf Binding", 270:23681-687 (1995).
Marble et al., "Peptides Block Ras Function; Potentially Blocks Oncogenic Development", Cancer Biotech Weekly, p. 6(1), (1996).
McGeady et al., "The Farnesyl Group of H-Ras Facilitates the Activation of a Soluble Upstream Activator of Mitogen-activated Protein Kinase", J. Biol. Chem., 270:26347-51 (1995).
Nassar et al., "The 2.2 .ANG. Crystal Structure of the Ras-binding Domain of the Serine/Threonine Kinase c-Raf-1 in Complex with Rap 1A and a GTP Analogue", Nature, 375:554-60 (1995).
Niehof et al., "A Small Peptide Derived from the Aminoterminus of c-Raf-1 Inhibits c-Raf-1/Ras Binding", Biochem. and Biophys. Res. Comm., 206:46-50 (1995).
Okada et al., "Post-translational Modification of H-Ras is Required for Activation of, but not for Association with, B-Raf", J. Biol. Chem., 271:4671-78 (1996).
Quest et al., "A Phorbol Ester Binding Domain of Protein Kinase C.gamma., Deletion Analysis of the Cys2 Domain Defines a Minimal 43-Amino Acid Peptide", J. Biol. Chem., 269:2961-70 (1994).
Vjotek et al., "Mammalian Ras Interacts Directly with the Serine/Threonine Kinase Raf", Cell, 74:205-14 (1993).
Warne et al., "Direct Interaction of Ras and the Amino-terminal Region of Raf-1 In Vitro", Nature, 364:352-55 (1993).
Yamamori et al., "Purification of a Ras-dependent Mitogen-activated Protein Kinase Kinase Kinase from Bovine Brain Cytosol and its Identification as a Complex of B-Raf and 14-3-3 Proteins", J. Biol. Chem., 270:11723-26 (1995).
Zhang et al., "Normal and Oncogenic p21.sup.ras Proteins Bind to the Amino-terminal Regulatory Domain of c-Raf-1", Nature, 364:308-13 (1993).

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