Influenza virus replicated in mammalian cell culture and vaccine

Details Influenza virus replicated in mammalian cell culture and vaccine Influenza virus replicated in mammalian cell culture and vaccine

1996-06-17

1998-10-20

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435239, 435325, C12N 500, C12N 702, C12N 700

Patent

active

058245367

ABSTRACT:
The invention provides replication of high growth influenza virus strains, derived from clinical isolates, in cultured mammalian cells by infecting the mammalian cells with the high growth strains to obtain infected cells, and culturing the cells while maintaining a trypsin concentration range of 0.05-1.0 .mu.g/ml in the culture medium, where the resulting replicated virus is suitable for use in mammalian influenza vaccines and vaccination methods, which are also provided by the invention.

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