Inflammation inducible hybrid promoters, vectors comprising...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C536S023400, C435S069100, C435S320100, C435S455000, C435S325000

Reexamination Certificate

active

06870044

ABSTRACT:
The present invention relates to the field of the regulation of the expression of genes and is based in particular on the use of constructs which make it possible to activate the transcription of a transgene in cells, tissues or organs under inflammatory conditions. The promoters of the invention preferably comprise a) a PPAR response element and b) the whole or part of the promoter of the PLA2sIIA gene. The invention also relates to vectors, cells and compositions comprising these promoters, as well as their use for the regulation of the expression of genes in vitro, ex vivo or in vivo.

REFERENCES:
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patent: WO 9821349 (1998-05-01), None
Cyril Couturier et al, Interleukin 1B Induces Type 2-secreted Phospholipase A2 Gene in Vascular Smooth Muscle Cells by a Nuclear Factor kB and Peroxisome Proliferator-activated Receptor-mediated Process, vol. 274, No. 33, Aug. 13, pp. 23085-23093, 1999.*
C. Couturier et al., Interleukin 1Beta Induces Type II-secreted Phospholipase A2 Gene in Vascular Smooth Muscle Cells by a Nuclear Factor KappaB and Peroxisome Proliferator-activated Receptor-mediated Process, J. Biol. Chem. 274(33):23085-23093 (1999).
C. Juge-Aubry et al., DNA Binding Properties of Peroxisome Proliferator-Activated Receptor Subtypes on Various Natural Peroxisome Proliferator Response Elements., J. Biol. Chem. 272(40):25252-25259 (1997).
K. Mukhopadhyay et al., Use of a New Rat Chondrosarcoma Cell line to Delineate a 119-Base Pair Chondrocyte-speciic Enhancer Element and to Define Active Promoter Segments in the Mouse Pro-alpha1(II) Collagen Gene, J. Biol. Chem. 270(46):27711-27719 (1995).
C. Massad et al., Induction of Secreted Type IIA Phospholipase A2 Gene Transcription by Interleukin-1Beta, J. Biol. Chem. 275(30):22686-22694 (2000).

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