Infectious bronchitis virus with an altered spike gene

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...

Reexamination Certificate

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C424S221100, C424S222100, C424S184100

Reexamination Certificate

active

07445785

ABSTRACT:
The present invention provides a vaccine for use in the protection of poultry against infectious bronchitis comprising an attenuated infectious bronchitis virus (IBV) and a pharmaceutical acceptable carrier or diluent, characterized in that the attenuated IBV comprises a heterologous spike gene. Such a vaccine is based on IBV strain Beaudette that is able to express a spike gone derived from a different IBV strain. The vaccines provided by the present invention also allow the administration via the in ovo route.

REFERENCES:
patent: WO 98/49195 (1998-11-01), None
patent: WO 01/64244 (2001-09-01), None
patent: WO 02/092827 (2002-11-01), None
Evans, et al. Utilising a defective IBV RNA for heterologous gene expression with potential phrophylactic application. Adv Exp. Med Biol. 1998; 440:687-92.
Niesters, et al. The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Vir. Res. 1986; 5(2-3): 253-63.
Cook, et al. A survey of the presence of a new infectious bronchitits virus designated 4/91 (793B). Veterin. Rec. 1996; 138(8):178-80.
Wang, Xiuqing et al. “Construction and Immunogenicity Studies of Recombinant Fowl Poxvirus . . . ” Avian Diseases (2002) 46:831-838.
Song, Chang-Seon, et al. “Induction of protective immunity in chickens vaccinated with infectious bronchitis virus S1 glycoprotein . . . ” J. of General Virology (1998) 79:719-723.
Wang, Li, et al. “Experimental confirmation of recombination upstream of the S1 hypervariable region of infectious bronchitis . . . ” Virus Research (1997) 49:139-145.
Casais, Rosa et al. “Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates . . . ” J. of Virology (Aug. 2003) V77, N16, p. 9084-9089.

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