Induction of embryogenesis using cytoskeleton inhibitors or prot

Details Induction of embryogenesis using cytoskeleton inhibitors or prot Induction of embryogenesis using cytoskeleton inhibitors or prot

1996-03-29

1999-05-04

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

435420, 435431, 435242, 47 58, C12N 500

Patent

active

059003758

ABSTRACT:
Embryogenesis from plant microspores is routinely induced with a 16-24 h temperature treatment of 32.5.degree. C. Continuous culture at 25.degree. C. results in pollen development. However, microspore treatment with anti-cytoskeletal agents, or protein synthesis inhibitors, at the non-inductive temperature of 25.degree. C., can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction. Furthermore, when anti-microtubule agents (e.g. colchicine) are used, embryo induction and chromosome doubling occur simultaneously, thus generating doubled haploids, whereas heat induction generates haploids. Thus, the use of microtubule inhibitors will provide a simple one-step process to simultaneously induce embryogenesis and chromosome doubling for the production of fertile plants, thus providing minimal manipulation which will be very advantageous for genetic studies and plant breeding programs. As noted, heat shock induces haploids. A low level of chromosome doubling can be obtained by adding colchicine to microspore cultures during the heat treatment. However, the use of trifluralin with the heat treatment, to generate doubled haploid plants results in an improved recovery of fertile doubled haploid plants than previously shown in the prior art.

REFERENCES:
W.A. Keller et al., Embryogenesis and Plant Regeneration in Brassica napus nther Cultures. Can. J. Bot. (1977) 55:1383-1388.
Astrid Gland et al., Genetic and Exogenous Factors Affecting Embryogenesis in Isolated Microspore Cultures of Brassica napus L. Plant Physiol, (1988) 132:613-617.
W.A. Keller et al., High Frequency Production of Microspore-derived Plants from Brassica napus Anther Cultures, Z. Pflanzenzuchtg, (1978) 80:100-108.
G. Wenzel et al., Anther Culture as a Breeding Tool in Rape, Z. Pflanzenzuchtg, (1977) 78:149-155.
M.C.M. Iqbal et al., Increased Embryogenesis after Colchicine Treatment of Microspore Cultues of Brassica napus L., J. Plant Physiol, (1994) 143:222-226.
M.A.M. Zaki et al., Microspore-derived Embyros in Brassica: The Significance of Division Symmetry in Pollen Mitosis I to Embryogenic Development. Sex Plant Reprod. (1991) 4:48-55.
Z.Z. Chen et al., Efficient Production of Doubled Haploid Plants through Chromosome Doubling of Isolated Microspores in Brassica napus. Plant Breeding, (1994) 113:217-221.
C. Mollers et al., Efficient Production of Doubled Haploid Brassica napus Plants by Colchicine Treatment of Microspores. Euphytica, (1994) 75:95-104.
Z. Fan et al., Development of Microspores in vivo and in vitro in Brassica napus L. Protoplasma. (1988) 147:191-199.
Phan V. Chuong et al., High Frequency Embryogenesis Through Isolated Microspore Culture in Brassica Napus L. and B. Carinata Braun. Plant Science, (1985) 39:219-226.
Regine Mathias et al., Effective Diploidization of Microspore-Derived Haploids of Rape (Brassica napus L.) by In Vitro Colchicine Treatment. Plant Breeding, (1991) 106:82-84.
N.C. Subrahmanyam et al., Chromosome Doubling of Barley Haploids by Nitrous Oxide and Colchicine Treatments. Can. J. Genet. Cytol. (1975) 17:573-583.
C.-S. Loh et al., The Response of Haploid Secondary Embryoids and Secondary Embryogenic Tissues of Winter Oilseed Rape to Treatment with Colchicine. New Phytol., (1983) 95:359-366.
B. Barnabas al., Direct Effect of Colchicine on the Microspore Embryogenesis to Produce Dihaploid Plants in wheat (Triticum aestivum L.). Theor. Appl. Genet., (1991) 81:675-678.
L.G. Burk et al., Diploidized Haploids From Aseptically Cultured Anthers of Nicotiana tabacum. The Journal of Heredity, 355-360.
Y. Wan et al., Efficient Production of Doubled Haploid Plants Through Colchicine Treatment of Anther-Derived Maize Callus. Theor. Appl. Genet., (1989) 77:889-892.

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