Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2005-06-28
2005-06-28
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S041000, C435S070400, C435S440000, C435S455000, C435S325000, C435S366000
Reexamination Certificate
active
06911324
ABSTRACT:
The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene and a NeuroD/BETA2 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are in contact with other cells in the culture, thereby inducing insulin gene expression in the cells. The invention also provides high throughput screening methods for modulators of β-cell function, stable cultures of cells made by the methods of the invention, and methods of treating a human subject using the methods of the invention.
REFERENCES:
patent: 5424286 (1995-06-01), Eng
patent: 5545618 (1996-08-01), Buckley et al.
patent: 5614492 (1997-03-01), Habener
patent: 5670360 (1997-09-01), Thorens
patent: 5705483 (1998-01-01), Galloway et al.
patent: 5723333 (1998-03-01), Levine et al.
patent: 5849989 (1998-12-01), Edlund
patent: 5858973 (1999-01-01), Habener et al.
patent: 5888816 (1999-03-01), Coon et al.
patent: 5977071 (1999-11-01), Galloway et al.
patent: 5981488 (1999-11-01), Hoffmann
patent: 6087129 (2000-07-01), Newgard et al.
patent: 6358739 (2002-03-01), Baetge et al.
patent: 6448045 (2002-09-01), Levine et al.
patent: 2003/0087394 (2003-05-01), Sharma
patent: WO 97/16536 (1997-05-01), None
patent: WO 00/09666 (2000-02-01), None
Beattie, G.M. et al. “Sustained proliferation of PDX-1+cells derived from human islets,”DiabetesMay 1999, pp. 1013-1019, vol. 48.
D'Ambra, R. et al. “Regulation of insulin secretion from β-cell lines derived from transgenic mice insulinomas resembles that of normal β-cells,”Endocrinol.1990, pp. 2815-2822, vol. 126, No. 6.
Dufayet De La Tour, D. et al. “β-cell differentiation from a human pancreatic cell line in vitro and in vivo,”Mol. Endocrinol.2001, pp. 476-483, vol. 15, No. 3.
Gouty Dominique
Itkin-Ansari Pamela
Levine Fred
Ketter James
Lambertson David
The Regents of the University of California
Townsend and Townsend / and Crew LLP
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