Induction-free process for the recombinant preparation of glutar

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4353201, 536 241, C12P 2102, C07H 2104, C12N 1556, C12N 1570

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active

057668817

ABSTRACT:
Improved, induction-free process for the recombinant preparation of glutarylamidase. The invention relates to a recombinant process for preparing glutarylamidase (GA), wherein the GA gene is expressed constitutively from an expression vector. In certain embodiments, the promoter for constitutively expressing the GA gene is a trc promoter or a trc-equivalent promoter which does not have any repressor or has a non-functional repressor.

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