Inducible alphaviral gene expression system

Details Inducible alphaviral gene expression system Inducible alphaviral gene expression system

2006-02-28

2006-02-28

Nguyen, Dave Trong (Department: 1635)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S325000, C435S252300, C424S218100, C536S023500, C536S023510, C536S023700, C536S024100

Reexamination Certificate

active

07005275

ABSTRACT:
The present invention provides novel expression vectors which permit tight regulation of gene expression in eucaryotic cells. More specifically, the invention provides DNA vectors comprising nucleotide sequences that are transcribed to form RNA molecules which are then replicated by a temperature-sensitive replicase to form additional RNA molecules. The RNA molecules produced by replication contain a nucleotide sequence which may be translated to produce a protein of interest or which encode one or more untranslated RNA molecules. Also provided are methods for producing heterologous proteins and untranslated RNA molecules. Further provided are methods for administering heterologous proteins and untranslated RNA molecules to individuals. In addition, pharmaceutical compositions are provided comprising the DNA and RNA molecules of the invention and a pharmaceutically acceptable carrier.

REFERENCES:
patent: 5091309 (1992-02-01), Schlesinger et al.
patent: 5217879 (1993-06-01), Huang et al.
patent: 5532154 (1996-07-01), Brown
patent: 5578473 (1996-11-01), Palese et al.
patent: 5580859 (1996-12-01), Felgner et al.
patent: 5705163 (1998-01-01), Pastan et al.
patent: 5756349 (1998-05-01), Lin
patent: 5766602 (1998-06-01), Xiong et al.
patent: 5789245 (1998-08-01), Dubensky, Jr. et al.
patent: 5792462 (1998-08-01), Johnston et al.
patent: 5814482 (1998-09-01), Dubensky, Jr. et al.
patent: 5843723 (1998-12-01), Dubensky, Jr. et al.
patent: 0 716 148 (1996-06-01), None
patent: WO 94/17813 (1994-08-01), None
patent: WO 96/17072 (1996-06-01), None
patent: WO 97/38087 (1997-10-01), None
patent: WO 98/36779 (1998-08-01), None
patent: WO 99/18226 (1999-04-01), None
Shirako et al (J. Virol. 72(3):2310-2315, Mar. 1998).
Suopanki et al. (J. Gen. Virol. (79:309-319, 1998).
Frolov et al al (J. Virol. 73(5):3854-3865, 1999).
Shirako et al (Virology 177:54-64, 1990).
Schnizer et. al., Mutations at Histidine 211 of the Yeast F1-ATPase B-Submit Affect the Stability and Assembly of the ATPase and the Structure of the Active Site, 1996; Archives of Biochemistry . . . , vol. 326: 126-136.
Rudinger (In Peptide Hormones, J.A. Parsons Ed. University Park press, 1996:1-7).
Hardy et al (Virology 177: 199-208, 1990).
Perri et al (J. Virol. 74(20): 9802-9807, 2000).
Agapov et al, Proc. Nat. Acad. Sci. USA 95: 12989-12994, se entire document, especially p. 12994, col. 1, first full paragraph, Oct. 1998.
Verma et al. Nature 389: 239-242, especially p. 239, Sep. 1997.
Anderson et al. Nature 392: 25-30, especially pp. 25 and 30, Apr. 1997.
Altman-Hamamdzic, S. et al.,“Expression of β-galactosidase in mouse brain: utilization of a novel nonreplicative Sindbis virus vector as a neuronal gene delivery system.”Gene Therapy 4:815-822 (Aug. 1997).
Artelt, P. et al., “Vectors for efficient expression in mammalian fibroblastoid, myeloid and lymphoid cells via transfection or infection,”Gene 68:213-219 (1988).
Artuc, M. et al., “Differential promoter activity in benign and malignant human cells of skin origin,”Exp. Dermatol. 4:317-321 (1995).
Barton, D. J. et al., “Demonstration In Vitro of Temperature-Sensitive Elongation of RNA in Sindbis Virus Mutant ts6,”J. Virol. 62:3597-3602 (1988).
Bechler, K., “Influence of Capping and Polyadenylation on mRNA Expression and on Antisense RNA Mediated Inhibition of Gene Expression,”Biochem. Biophys. Res. Comm. 241:193-199 (Dec. 1997).
Berglund, P. et al., “Alphaviruses as vectors for gene delivery,”TIBTECH 14:130-134 (Apr. 1996).
Bohl, D. et al., “Long-term control of erythropoietin secretion by doxycycline in mice transplanted with engineered primary myoblasts,”Nature Med. 3:299-305 (Mar. 1997).
Bredenbeek, P. J. and C. M. Rice, “Animal RNA virus expression systems,”Seminars in Virol. 3:297-310 (1992).
Bredenbeek, P. J. et al., “Sindbis Virus Expression Vectors: Packaging of RNA Replicons by Using Defective Helper RNAs,”J. Virol. 67:6439-6446 (1993).
Burge, B. W. and E. R. Pfefferkorn, “Isolation and Characterization of Conditional-Lethal Mutants of Sindbis Virus,”Virol. 30:204-213 (1966).
Burge, B. W. and E. R. Pfefferkorn, “Complementation Between Temperature-sensitive Mutants of Sindbis Virus,”Virol. 30:214-223 (1966).
Chapman, K. B. and J. W. Szostak, “Isolation of a ribozyme with 5′-5′ Ligate activity,”Chem.&Biol. 2:325-333 (1995).
Crameri, A. et al., “Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling,”Nature Biotech. 14:315-319 (Mar. 1996).
Davis, N. L. et al., “In Vitro Synthesis of Infectious Venezuelan Equine Encephalitis Virus RNA from a cDNA Clone: Analysis of a Viable Deletion Mutant,”Virol. 171:189-204 (1989).
De Groot, R. J. et al., “Sindbis virus RNA polymerase is degraded by the N-end rule pathway,”Proc. Natl. Acad. Sci. USA 88:8967-8971 (1991).
Doedens, J. R. et al., “Inhibition of Endoplasmic Reticulum-to-Golgi Traffic by Poliovirus Protein 3A: Genetic and Ultrastructural Analysis,”J. Virol. 71:9054-9064 (Dec. 1997).
Dryga, S. A. et al., “Identification of Mutations in a Sindbis Virus Variant Able to Establish Persistent Infection in BHK Cells: The Importance of a Mutation in the nsP2 Gene,”Virol. 228:74-83 (Feb. 1997).
Dubensky, Jr., T. W. et al., “Sindbis Virus DNA-Based Expression Vectors: Utility for In Vitro and In Vivo Gene Transfer,”J. Virol. 70:508-519 (Jan. 1996).
Dubuisson, J. et al., “Formation and Intracellular Localization of Hepatitis C Virus Envelope Glycoprotein Complexes Expressed by Recombinant Vaccinia and Sindbis Viruses,”J. Virol. 68:6147-6160 (1994).
Frolov, I. et al., “Alphavirus-based expression vectors: Strategies and applications,”Proc. Natl. Acad. Sci. USA93:11371-11377 (Oct. 1996).
Früh, K. et al., “Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex,”EMBO J. 13:3236-3244 (1994).
Furth, P. A. et al., “Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter,”Proc. Natl. Acad. Sci. USA 91:9302-9306 (1994).
Gossen, M. and H. Bujard, “Tight control of gene expression in mammalian cells by tetracycline-responsive promoters,”Proc. Natl. Acad. Sci. USA 89:5547-5551 (1992).
Hahn, C. S. et al., “Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation,”Proc. Natl. Acad. Sci. USA 89:2679-2683 (1992).
Hakimi, J. and P. H. Atkinson, “Glycosylation of Intracellular Sindbis Virus Glycoproteins,”Biochem. 21:2140-2145 (1982).
Hariharan, M. J. et al., “DNA Immunization against Herpes Simplex Virus: Enhanced Efficacy Using a Sindbis Virus-Based Vector,”J. Virol. 72:950-958 (Feb. 1998).
Hennighausen, L. et al., “Conditional Gene Expression in Secretory Tissues and Skin of Transgenic Mice Using the MMTV-LTR and the Tetracycline Responsive System,”J. Cell. Biochem. 59:463-472 (1995).
Herweijer, H. et al., “A Plasmid-Based Self-Amplifying Sindbis Virus Vector,”Human Gene Ther. 6:1161-1167 (1995).
Hoffmann, A. et al., “A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines,”Nucl. Acids Res. 25:1078-1079 (Mar. 1997).
Howe, J. R. et al., “The Responsiveness of a Tetracycline-sensitive Expression System Differs in Different Cell Lines,”J. Biol. Chem. 270:14168-14174 (1995).
Huang, H. V., “Sindbis virus vectors for expression in animal cells,”Curr. Opin. Biotech. 7:531-535 (Oct. 1996).
Jeng, S.-Y. et al., “Characterization and Partial Purification of Bovine α-Lactalbumin and β-Casein Produced in Milk of Transgenic Mice,”J. Dairy Sci. 80:3167-3175 (Dec. 1997).
Johanning, F. W. et al., “

Affiliated with

Also associated with

Rating

Inducible alphaviral gene expression system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Inducible alphaviral gene expression system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Inducible alphaviral gene expression system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3660396