Chemistry: analytical and immunological testing – Involving producing or treating antigen or hapten – Producing labeled antigens
Patent
1986-08-13
1991-12-10
Kepplinger, Esther L.
Chemistry: analytical and immunological testing
Involving producing or treating antigen or hapten
Producing labeled antigens
436501, 436504, 436518, 436538, 436542, 436 56, 436 57, 435 6, 935 78, G01N 33534, G01N 33566, G01N 33567, C12Q 168
Patent
active
050717758
ABSTRACT:
A sensitive and general method of post-separation detection and quantification of chemical compounds is described. The method involves separation of unlabeled compounds by chromatography, electrophoresis or other means and selective binding of the separated compounds to ligands containing highly neutron-activatable elements, followed by neutron irradiation. The neutron-activatable elements are converted to their radiation-emitting isotopes by neutron absorption, and detection is done by autoradiography, fluorography or other means of radiation detection. The theoretical sensitivity of the method is in the attomole (10.sup.-18 mole) range.
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Bernard, Jr. John A.
Harling Otto R.
Kwok Kwan S.
Snapka Robert M.
Varshavsky Alexander
Cook Paul J.
Graeter Janelle
Kepplinger Esther L.
Massachusetts Institute of Technology
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