Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is particulate and the particles are of...
Patent
1994-02-07
1995-10-24
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is particulate and the particles are of...
435 725, 435971, 436 70, 436164, 436165, 436514, 436518, 436528, 436531, 436534, 436536, 436538, 436541, 436805, 436810, 436824, 436829, G01N 33538, G01N 33546
Patent
active
054609798
ABSTRACT:
A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.
REFERENCES:
patent: 4480038 (1984-10-01), Cheng
patent: 4721681 (1988-01-01), Lentrichia et al.
patent: 4940668 (1990-07-01), Wardlaw et al.
Pharmacia LKB Biotechnology Products, 1988.
Levine Robert A.
Mercolino Thomas J.
Recktenwald Diether J.
Terstappen Leon W. M. M.
Wardlaw Stephen C.
Becton Dickinson and Company
Chin Christopher L.
Jones William W.
Scheiner Toni R.
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