Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-01-12
1990-09-04
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
356320, 356341, 356407, 435 25, 435 28, 436518, 436527, 436531, 436534, 436537, 436544, 436805, 436811, 436817, 436818, G01N 3353, G01N 33543, C12Q 128, G01J 342
Patent
active
049544357
ABSTRACT:
The method of the present invention employs an enzyme immunoassay for measuring the concentration of an analyte in a sample by indirect colorimetric detection. An incident light beam at a plurality of wavelengths is directed into a liquid solution containing an analyte of interest. The solution is capable of attenuating the amount of light at a first wavelength received from this solution as a function of the increasing concentration of the analyte present. A light signal from the solution at the first wavelength is detected, and light at a second wavelength, at which substantially no attenuation of light signal occurs as the concentration of the analyte increases, is also detected. The ratio of the two respective wavelengths is formed and that ratio is compared with ratios of known amounts of the analyte to determine the amount of the analyte in the sample.
REFERENCES:
patent: 3527538 (1965-08-01), Allen et al.
patent: 3781112 (1973-12-01), Groner et al.
patent: 3813168 (1974-05-01), Honkawa
patent: 4495273 (1985-01-01), Shaffar
patent: 4503143 (1985-05-01), Gerber et al.
patent: 4680274 (1987-07-01), Sakai et al.
patent: 4680275 (1987-07-01), Wagner et al.
patent: 4714672 (1987-12-01), Rokugawa et al.
patent: 4743561 (1988-05-01), Shaffar
"A Nephelometry System for the Abbot TDx.RTM. Analyzer" R. F. DeGrella et al, Clinical Chemistry 31(9); 1474-77; 1985.
"3,3',5,5'-Tetramethylbenzidine as an Ames Test Negative Chromogen for Horse-Radish Peroxidase in Enzyme-Immunoassay", E. S. Bos et al, J. of Immunoassay, 2(3 & 4); 187-204, 1981.
Becton Dickinson and Company
Hoffer Florina B.
Kepplinger Esther L.
Passman Aaron
LandOfFree
Indirect colorimetric detection of an analyte in a sample using does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Indirect colorimetric detection of an analyte in a sample using , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Indirect colorimetric detection of an analyte in a sample using will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-441634