Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1996-12-31
1998-12-22
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 5, 435325, 435975, C12N 510, C12Q 102, C12Q 104, C12Q 170
Patent
active
058517570
ABSTRACT:
Cell lines and methods are disclosed for detecting the presence of RNA viruses in a specimen. The cell lines are stably transformed with a DNA molecule that includes a promoter capable of being recognized by the DNA dependent RNA polymerase of the cell capable of directing the transcription of a cDNA of a structurally defective RNA virus genome operably coupled to the promoter. The cDNA contains a structural coding sequence encoding a selected reporter gene product. The RNA molecules transcribed by the DNA dependent RNA polymerase are not capable of causing the translation of the reporter gene in the cell except when an active related virus that provides the necessary trans-acting enzymes to cause the increased replication of the RNA containing the reporter gene which is then translated into the reporter gene product is provided. Methods utilizing the cell lines of this invention to detect RNA viruses in a specimen by incubating the specimen with the cell line and assaying for expression of the reporter gene and a kit containing a supply of the cells and a supply of the reagents necessary for the detection of the reporter gene product are also provided.
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Olivo Paul D.
Schlesinger Sondra
Railey II Johnny F.
Washington University
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