Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-28
1996-04-16
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435810, 536 231, 536 2433, 536 253, 935 1, 935 8, 935 16, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2102, C12N 1500
Patent
active
055081690
ABSTRACT:
Synthetic nucleic acid molecules are provided which are called indexing linkers and which can be selectively linked to unknown, or non-identical cohesive ends of nucleic acid fragments. Such indexing linkers are useful for the selective isolation, identification, amplification, labelling, and modification, of nucleic acid fragments, especially subsets of such fragments released by cleavage using Type IIS restriction endonucleases or restriction endonucleases recognizing interrupted palindromic sequences. Sets of such indexing linker molecules are described which are useful for indexing fragments released by cleavage of a genome. Methods are described for distinguishing such fragments using said indexing linker molecules for effecting detection, or further modification of such fragments; and for amplifying such fragments by the polymerase chain reaction.
REFERENCES:
patent: 4719179 (1988-01-01), Barany
patent: 4935357 (1990-06-01), Saybalski
New England BioLabs.RTM. 1988-1989 Catalog, p. 63.
Mormeneo et al., "Precise Nucleotide Sequence Modifications with Bidirectionally cleaving Class IIS excision Linkers", Gene, vol. 61, No. 1, 1987, pp. 21-30; Abstract Only.
Sommer and Tautz, Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6749.
Deugau Kenneth V.
Unrau Paul
Atomic Energy of Canada Limited
Jones W. Gary
Queen's University at Kingston
Sisson Bradley L.
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