Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Reexamination Certificate
2007-04-03
2008-10-07
Mondesi, Robert B. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
C435S052000, C435S056000
Reexamination Certificate
active
07432075
ABSTRACT:
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO:1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof.The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9α-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.
REFERENCES:
patent: WO 01/31050 (2001-05-01), None
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Van der Geize et al., “Unmarked gene deletion mutagenesis of kstD, encoding 3-ketosteroid Δ1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker,” FEMS Microbiology Letters 205, pp. 197-
Dijkhuizen Lubbert
Hessels Gerda
Van der Geize Robert
Van der Meijden Peter
Gebreyesus Kagnew
Hess Susan
Mondesi Robert B.
N.V. Organon
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