Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1994-08-15
1998-08-11
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 800255, 800DIG24, 800DIG26, 800DIG27, 800DIG43, 800DIG45, 800DIG25, 800DIG52, 800DIG56, 4352404, 435 691, 4351723, 536 237, 536 241, C12N 1529, C12N 1582, A01H 400, A01H 500
Patent
active
057929211
ABSTRACT:
Two nucleotide sequences encoding two different polypeptides found in yeast trehalose synthase have been isolated and cloned. A third polypeptide has been isolated from the enzyme and characterized, and a method is provided to isolate and clone the nucleotide sequence encoding this polypeptide. The coding sequences can be inserted into suitable vectors and used to transform host cells. The transformed cells will produce increased amounts of trehalose compared to the untransformed wild types and have increased tolerance to a variety of stresses, in particular to decreased availability of water. The invention may be used to improve the stress tolerance of organisms, to increase the storage life of foodstuffs and to produce trehalose economically on an industrial scale in an organism (e.g, baker's yeast) that is a traditional and safe foodstuff.
REFERENCES:
Naploi et al. Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. The Plant Cell, vol. 2 279-289, Apr. 1990.
Veluthambi et al. Trehalose Toxicity in Cuscuta reflexa. Plant Physiol. (1981) 68, 1369-1374.
Holmstrom Kjell-Ove
Londesborough John
Mandal Abul
Palva Tapio
Tunnela nee Vuorio Outi
Haas Thomas
Robinson Douglas W.
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