Increasing the sensitivity in the immunochemical determination o

Details Increasing the sensitivity in the immunochemical determination o Increasing the sensitivity in the immunochemical determination o

1997-07-17

2000-02-29

Stucker, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 792, 435 794, 435 795, 435962, 435974, 435975, 436518, 436536, 436538, 436540, C12Q 170

Patent

active

060307706

ABSTRACT:
The present invention relates to a process for the determination of an analyte, in which a sample optionally containing the analyte to be determined is brought into contact with a first receptor R1, which has a binding affinity for the analyte, immobilized on a solid phase, and a receptor R2, which likewise has a binding affinity for the analyte, is added and in which furthermore the resulting immune complexes are brought into contact with a binding factor which has a binding affinity for the analyte and for a receptor R3 immobilized on the same or a second solid phase and in which the amount of the analyte bound to the first solid phase or, if present, to the second solid phase is detected in a suitable manner.

REFERENCES:
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4495296 (1985-01-01), Neurath et al.
patent: 4743542 (1988-05-01), Graham, Jr. et al.
Seiichi Hashida et al., "Detection of Antibody IgG to HIV-1 in Urine by Ultrasensitive Enzyme Immunoassay (Immune Complex Transfer Enzyme Immunoassay) Using Recombinant p24 as Antigen for Diagnosis of HIV-1 Infection", Journal of Clinical Laboratory Analysis, pp. 8:86-95, 1994.

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