Increasing expression of transgenes in plant cells using insulat

Details Increasing expression of transgenes in plant cells using insulat Increasing expression of transgenes in plant cells using insulat

1999-01-08

2000-08-08

Benzion, Gary

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

800295, 800298, 435468, 435419, 4353201, 536 236, 536 241, 536 237, C12N 1500, C12N 1529, C12N 1582, A01H 300

Patent

active

061004480

ABSTRACT:
A method of making recombinant plant cells having reduced variability of expression of foreign genes therein is described herein. The method comprises (a) providing a plant cell capable of regeneration; and (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and an insulator (or "boundary element") positioned (i) 5' to the transcription initiation region, (ii) 3' to the structural gene, or (iii) both 5' to the transcription initiation region and 3' to the structural gene. DNA constructs useful for carrying out the method and plant cells and plants produced by the method are also disclosed.

REFERENCES:
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V. G. Corces, "Keeping enhancers under control", Nature, 376 462-463 (1995).
Cal, et al., "Modulation of enhancer-promoter Interactions by insulators in the Drosophila embryo", Nature 376 533-536 (1995).
Allen, et al., "High-Level Transgene Expression in Plant Cells: Effects of a Strong Scafold Attachment Region from Tobacco" The Plant Cell 8 899-913 (1996).
Allen, et al., "Scaffold Attachment Regions Increase Reporter Gene Expression in Stably Transformed Plant Cells" The Plant Cell 5 603-613 (1993).
Kellum et al., "A position-effect assay for boundaries of higher order chromosomal domains" Cell 64:941-950 (Mar. 1991).
Mlynarova et al., "Reduced position effect in mature transgenic plants conferred by the chicken lysozyme matrix-associated region" The Plant Cell 6:417-426 (Mar. 1994).
Farkas et al., "Sequence of scs and scs' Drosophila DNA fragments with boundary function in the control of gene expression" Nucleic Acids Research 20:2604 (1992).

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