Increased throughput analysis of small compounds using multiple

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 6, 204450, 204453, 204456, B01D 5702, B01D 5942, C12Q 168

Patent

active

061561789

ABSTRACT:
The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.

REFERENCES:
Pigge et al., "Capillary electrophoresis for the detection of known point mutations by single-nucleotide primer extension and laser-induced fluorescence detection," Journal of Chromatography A, 1997, vol. 781, pp. 367-375.
Mansfield et al., "Analysis of multiplexed short tandem repeat (STR) systems using capillary array electrophoresis," Electrophoresis, 1998, vol. 19, pp. 101-107.
Mansfield et al., "Rapid sizing of polymorphic microsatellite markers by capillary array electrophoresis," Journal of Chromatography A, 1997, vol. 781, pp. 295-305.
Li et al., "Effects of gel material on fluoresce lifetime detection of dyes and dye-labeled DNA primers in capillary electrophoresis," Journal of Chromatography A, 1999, vol. 841, pp. 95-103.
Zhu et al., "High-Sensitivity Capillary Electrophoresis of Double-Stranded DNA Fragments Using Monomeric and Dimeric Fluorescent Intercalating Dyes," 1994, vol. 66, No. 13, pp. 1941-1948.
Warren et al., "Principles and methods for the Analysis and purification of synthetic deoxyribonucleotides by High-Performance Liquid Chromatography," Molecular Biotechnology, 1995, vol. 4, pp. 179-199.
Nau et al., "A microbotics approach to LC sample preparation," Am. Lab. 1992, vol. 24, No. 3, pp. 64, 66-68.
Gerino et al., "Comparison of different tracers and methods used to quantify bioturbation during a spring bloom: 234-Thorium, luminophores and chlorophyll alpha," Estuarine Coastal and Shelf Science, 1998, vol. 46, No. 4, pp. 531-547.
Effenhauser et al., "Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights," Analytical Chemistry, 1993, vol. 65, No. 19, pp. 2637-2642.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Increased throughput analysis of small compounds using multiple does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Increased throughput analysis of small compounds using multiple , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Increased throughput analysis of small compounds using multiple will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-958046

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.