Increased production of Thermus aquaticus DNA polymerase in E. c

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

43525233, 435 914, 435 9142, 435 691, 435 697, 4353201, 536 231, 536 232, C12Q 168, C12N 121, C12N 1511, C12N 1563

Patent

active

060836865

ABSTRACT:
The Thermus aquaticus gene encoding a thermostable DNA polymerase (Taq Pol) is altered in the N-terminus-encoding region to provide mutant genes with improved expression in E. coli.

REFERENCES:
patent: 4703004 (1987-10-01), Hopp et al.
patent: 4782137 (1988-11-01), Hopp et al.
patent: 4889818 (1989-12-01), Gelfand et al.
Saiki et al. (1988), Science 239: 487-491.
Grosjean et al. (1982), Gene 18: 199-209.
Gerard et al., Biochemistry (1990), vol. 29, pp. 9274-9281. Construction and Expression of a Novel Recombinant Anaphylatoxin, C5a-N19, as a Probe for the Human C5a Receptor.
Hopp et al., Bio/Technology (1988), vol. 6, pp. 1204-1210. A short Polypeptide Marker Sequence Useful For Recombinant Protein Identification and Purification.
Bonekamp et al., Nucleic Acids Research (1988), vol. 16, No. 7. The AGG codon is translated slowly in E.coli even at very low expression levels.
Lee et al., Gene (1987), vol. 58, pp. 77-86. Modification of mRNA secondary structure and alteration of the expression of human interferon .alpha.1 in Escherichia coli.
Lawyer et al., (1989), Journal of Biological Chemistry, vol., 264, No. 11, Issue of Apr. 15, pp. 6427-6437. Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus.
Pricket et al., (1989), BioTechniques, vol. 7, No. 6. A Calcium-Dependent Antibody for Identification and Purification of Recombinant Proteins.
Isacchi, A. et al, (1989), Mature Apolipoprotein A1 and its Precursor, proApoA1: Influence of the Sequence at the 5' end of the Gene on the Efficiency of Expression in Escherichia coli., Gene 81:129-137.
Looman, A. C. et al., (1987), Influence of the Codon Following the AUG Initiation Codon on the Expression of a Modified lacZ Gene in Escherichia coli., EMBO Journal 6:2489-2492.
Hsiung, H. M. et al, (1987), Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone by Cathepsin C Cleavage, Methods Enzymol. 153:390-401.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Increased production of Thermus aquaticus DNA polymerase in E. c does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Increased production of Thermus aquaticus DNA polymerase in E. c, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Increased production of Thermus aquaticus DNA polymerase in E. c will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1484889

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.