Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-08-09
2005-08-09
Achutamuthy, P. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S320100, C435S252300, C435S252320, C435S254110, C435S325000, C435S419000, C536S023200, C536S023700
Reexamination Certificate
active
06927046
ABSTRACT:
The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. In a preferred embodiment, the invention provides methods to increase the production of L-lysine inCorynebacterium glutamicumby way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine inCorynebacterium glutamicumby way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes ofCorynebacterium glutamicumsuch as a naturally occurring, feedback-sensitive form of aspartokinase (ask) resulting from a threonine to isoleucine mutation at amino acid residue380in the ask gene of ATCC 21529, aspartate-semialdehyde dehydrogenase (asd), dihydrodipicolinate synthase (dapA), dihydrodipicolinate reductase (dapB), diaminopimelate dehydrogenase (ddh), and diaminopimelate decarboxylase (lysA).
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Crafton Corey M.
Hanke Paul D.
Li-D'Elia Lhing-Yew
Rayapati P. John
Walsh Holly J.
Achutamuthy P.
Archer-Daniels-Midland Company
Buchanan & Ingersoll PC
Cochenour Craig G.
Kerr Kathleen
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