Increased expression of a gene by a second transferred mammary g

Details Increased expression of a gene by a second transferred mammary g Increased expression of a gene by a second transferred mammary g

1995-06-06

1998-02-03

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4353171, 935111, 800 2, C12N 1563, C12N 1516, C12N 1579

Patent

active

057143455

ABSTRACT:
Transgenic animals capable of expressing a desired protein can be prepared by co-introducing into an egg or embryo cell of an animal, a first sequence, which encodes the desired protein, and a second, more efficiently expressed, DNA sequence. Expression efficiency is thereby conferred on the first sequence, leading to improved yield or targeting, or both. Co-introduction may be achieved by co-injecting a mixture of the two DNA sequences into a fertilized egg, in the case of an animal. The invention can be used to enhance the efficiency of expression of desired proteins, such as those having pharmaceutical activity in the mammary gland of a female transgenic animal.

REFERENCES:
patent: 5221778 (1993-06-01), Byrne et al.
patent: 5322775 (1994-06-01), Clark et al.
Kappel et al., Curr. Opin. Biotech. 3:548-53 (1992).
Clark et al., Biotechnology 7:487-492 (1989).
Archibald et al., Proc. Natl. Acad. Sci. USA, 87:5178-5182 (1990).
Wilmut et al., News Scientist, 7 Jul. 1988, pp. 56-59.
Van Brunt, BioTechnology 6(10):1149-1154 (1988).
Wilmut et al., "Production of pharmaceutical proteins in milk", Experientia 47: 905-912 (1991).

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