Increased efficiency of electrophoretic separations by mobility

Details Increased efficiency of electrophoretic separations by mobility Increased efficiency of electrophoretic separations by mobility

1994-12-20

1997-03-25

Phasge, Arun S.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204468, 204549, 204645, G01N 2726

Patent

active

056140720

ABSTRACT:
The invention concerns electrophoresis and methods and electrolyte compositions for decoupling or isolating the dual functions of the electrolyte composition in order to increase separation selectivity or reduce electromigration dispersion, or both. An electrolyte composition is used in electrophoresis to separate a charged analyte having a mobility .mu..sub.a in an electric field. The composition includes a buffer system having an ionic component that controls the pH of the composition and mobility matching ions having a mobility .mu..sub.2 in the electric field, such that .mu..sub.a /.mu..sub.2 equals about one. Alternatively, the buffer system may maintain the composition's pH and a complexing agent may complex with the buffer system to alter the mobility .mu..sub.2 of an ionic component of the buffer system, such that .mu..sub.a /.mu..sub.2 equals about one. The pH of the composition is within a pH range selected based on the analyte to be separated, and .mu..sub.2 remains substantially constant over this pH range.

REFERENCES:
patent: 5192405 (1993-03-01), Peterson et al.
M. Stefansson & M. Novotny, Anal. Chem. 1994, 66, 3466-3471.
Y. Rawjee, and Gy. Vigh, A Peak Resolution Model for the Capillary Electrophoretic Separation of the Enantiomers of Weak Acids with Hydroxypropyl .beta.-Cyclodextrin-Containing Background Electrolytes, 66 Anal. Chem. 619-627 (1994).
Y. Rawjee, R. Williams, and Gy. Vigh, Capillary Electrophoretic Chiral Separations Using Cyclodextrin Additives, 680 J. Chromatogr. 599-607 (1994).
Y. Rawjee, D. Staerk, and Gy. Vigh, Capillary Electrophoretic Chiral Separations With Cyclodextrin Additives, 635 J. Chromatogr., 291-306 (1993).
Y. Rawjee, R. Williams, and Gy. Vigh, Capillary Electrophoretic Chiral Separations Using .beta.-Cyclodextrin as Resolving Agent, 652 J. Chromatogr., 233-245 (1993).
R. Kuhn, and S. Hoffstetter-Kuhn, Chiral Separation by Capillary Electrphoresis, 34 Chromatographix 505-512 (1992).
E. Dose, and G. Guiochon, High-Resolution Modeling of Capillary Zone Electrophoresis and Isotachophoresis, 63 Anal. Chem. 1063-1072 (1991).
V. Sustacek, F. Foret, and P. Bocek, Selection of the Background Electrolyte Composition With Respect to Electromigration Dispersion and Detection of Weakly Absorbing Substances in Capillary Zone Electrophoresis, 545 J. Chromatogr. 239-248 (1991).
S. Terabe, Electrokinetic Chromatography: An Interface Between Electrophoresis and Chromatography, 8 Trends in Anal. Chem. 129-134 (1989).
J. Jorgenson, Zone Electrophoresis in Open-Tubular Capillaries, 3 Trends in Anal. Chem. 51-54 (1984).
T. Hirokawa, M. Nishino, N. Aoki, Y. Kiso, Y. Sawamoto, T. Yagi, J-L. Akiyama, Table of Isotachophoretic Indices, 271 J. Chromatogr. D1-D10 (1983).
J. Jorgenson and K. Lukacs, Zone Electrophoresis in Open-Tubular Glass Capillaries, 53 Anal. Chem. 1298-1302 (1981).
F. Mikkers, F. Everarts, and T. Verheggen, Concentration Distributions in Free Zone Electrophoresis, 169 J. Chromatogr. 1-10 (1979).
F. Everaerts, J. Beckers and T. Verheggen, Isotachophoresis Theory, Instrumentation and Applications, 6 J. Chromatgr. 27-40 (1976).
E. Dismukes and R. Alberty, Weak Electrolyte Moving Boundary Systems Analogous to the Electrophoresis of a Single Protein, 76 J. Amer. Chem. Soc., 191-197 (1954).
R. Alberty, Moving Boundary Systems Formed By Weak Electrolytes. Theory of Simple Systems Formed by Weak Acids and Bases, 72 J. Amer. Chem. Soc., 2361-2374 (1950).
V. Dole, A Theory of Moving Boundary Systems Formed by Strong Electrolytes, 67 J. Amer. Chem. Soc., 1119-1126 (1945).

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