Incapacitated whole-cell immunogenic bacterial compositions

Details Incapacitated whole-cell immunogenic bacterial compositions Incapacitated whole-cell immunogenic bacterial compositions

2005-07-05

2005-07-05

Graser, Jennifer E. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C424S243100, C424S257100, C424S256100, C424S249100, C424S259100, C424S093100, C424S093200, C424S093400, C424S093600, C435S252300, C435S320100

Reexamination Certificate

active

06913753

ABSTRACT:
The invention features incapacitated whole cell bacterial immunogenic compositions produced by infecting a bacterium with Lys minus bacteriophage, which are deficient in the lysin protein. Lys minus bacteriophage retain activity in infection of its appropriate bacterial host, destruction of the bacterial genome, and replication, which are sufficient to inhibit bacterial growth and replication. The resulting, Lys minus-infected bacterium is provided in a state of bacteriostasis, and is not capable of replicating further (e.g., is “incapacitated”). The incapacitated bacterium can then be used as to elicit an immune response for prophylactic and/or therapeutic purposes. The invention thus also features incapacitated bacteria formulated appropriately for use in immunogenic compositions for eliciting an immune response, e.g., for production of antibodies in a non-human host or in a whole cell bacterial vaccine.

REFERENCES:
patent: 4376110 (1983-03-01), David et al.
patent: 6130082 (2000-10-01), Majararian et al.
Longchamp et al. 1996. Abstracts of the Gen.Meet. of Amer.Soc. for Microbiol., May 19-23, p. 576.
Devine et al. J.Bacteriol. 1977. 129(2): 1072-1077.
Grundling et al. J.Bacteriol. Nov. 2000. 182(21): 6082-6090.
The American Heritage Dictionary of the English Language. 4thedition. 2000. entry “incapacitated”.
Ackermann, “Tailed bacteriophages: the order caudovirales,”Adv Virus Res, 51:135-201. (1998).
Arendt et al., Molecular Characterization of Lactorcoccal Bacteriophage Tuc2009 and Identification and Analysis of Genes Encoding Lysin, a Putative Holin, and Two Structural Proteins, Applied and Environment Microbiology, Jun. 1994, p. 1875-1883, vol. 60, No. 6.
Auad et al., “Physical mapping and partial genetic characterization of theLactobacillus delbrueckiisubsp. bulgaricus bacteriophage lb539,”Arch Virol, 144: 1503-1512. (1999).
Boizet et al., “Cloning, expression and sequence analysis of an endolysin-encoding gene ofLactobacillus bulgaricusbacteriophage mv1.”Gene, 94: 61-67 (1990).
Botstein et al., “Strategies and Applications of in Vitro Mutagenesis,” Science 229, vol. 229, p. 1193-1201, No. 4719 (1985).
Calandra et al., “Cellular streptolysin S-related hemolysins of group A Streptococcus C203S,”Infect Immun, 12: 13-28. (1975).
Calandra et al., “Lysis and protoplast formation of group B streptococci by mutanolysin,”Infect Immun, 28: 1033-1037 (1980).
Caldentey et al., The Lytic Enzyme of the Pseudommonas Phage Φ6. Purification and Biochemical Characterization, Biochimica et Biophysica Acta, 1159, 44-50 (1992).
Cattozzo et al., “Expression and Immunogenicity of V3Loop Epitopes of HIV-1, Isolates SC and WMJ2, Inserted in Salmonella Flagellin,” Journal Biotechnology 56 (1997) 191-203.
Chalfie et al., “Green Fluorescent Protein as a Marker for Gene Expression,” Science, vol. 263, 802-805 1994.
Chandry et al., “Analysis of the DNA sequence, gene expression, origin of replication and modular structure of theLactococcus lactislytic bacteriophage sk1,”Mol. Microbiol, 26: 49-64 (1997).
Cohen et al., “Simple procedure for production by group C streptococci of phage- associated lysin active against group A streptococci,”Appl Microbiol, 29: 175-178 (1975).
Cole et al., “The EBV-hybridoma technique and its application to human lung cancer,” Monoclonal Antibodies And Cancer Therapy, Alan R. Liss, Inc., pp. 77-96 (1985).
Cote et al., “Generation of human monoclonal antibodies reactive with cellular antigens,” Proc. Natl. Acad. Sci. USA 80: 2026-2030 (1983).
Coleman et al., “Cloning and expression inEscherichia coliandStraphylococcus aureusof the beta-lysin determinant fromStaphylococcus aureus: evidence that bacteriophage conversion of beta-lysin activity is caused by insertional inactivation of the beta-lysin determinant,” Microb Pathog, 1: 549-564 (1986).
Coleman et al.,Staphylococcus aureusbacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and enterotoxin A: molecular mechanism of triple conversion.J Gen Microbiol, 135.
Cooney et al., “Molecular cloning and genetic analysis of the determinant for gamma- lysin, a two-component toxin of Staphylococcus aureus,”J Gen Microbiol, 134:2179-2188 (1988).
Cormack, et al., “FACS-optimized mutants of the green fluorescent protein (GFP),” Gene, 173, 33-38 (1996).
de Ruyter et al., “Food-grade controlled lysis ofLactococcus lactisfor accelerated cheese ripening,”Nat Biotechnol, 15: 976-979 (1997).
Diaz et al., “The two-step lysis system of pneumococcal bacteriophage EI-1 is functional in gram-negative bacteria: triggering of the major pneumococcal autolysin inEscherichia coli,” Mol Microbiol, 19: 667-681.
Dietrich et al., “Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicideListeria monocytogenes,” Nat Biotechnol, 16: 181-185 (1998).
Elias et al., “Staphylococcus aureushaemolysins: their use in strain typing,”Acta Microbiol Acad Sci Hung, 27: 183-190 (1980).
Fischetti et al., “Purification and physical properties of group C streptococcal phage- associated lysine,”J Exp Med, 133: 1105-1117 (1971).
Gaeng et al., “Gene cloning and expression and secretion of listeria monocytogenes bacteriophage-lytic enzymes in lactococcus lactis,” Appl. Environ. Microbiol. 66, 2951 (2000).
Garcia et al., “Biochemical characterization of a murein hydrolase induced by bacteriophage Dp-1 inStreptococcus pneumoniae: comparative study between bacteriophage-associated lysin and the host amidase,”J.
Garcia et al., “Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin,”J Virol, 61: 2573-2580 (1987).
Garcia et al., “Mechanism of phage-induced lysis in pneumococci.” J Gen Microbiol, 129: 479-487. (1983).
Garrett, “Cell Lysis by induction of cloned lambda lysis genes,” J. et al. Mol. Gen. Genet. 182, 326 (1981).
Garvey et al., “Nucleotide sequence of bacillus phage Φ29 genes 14 and 15: homology of gene 15 with other phage lysozymes,” Nucleic Acids Res. 14, 10001 (1986).
Gindreau et al., “Molecular analysis of the region encoding the lytic system fromOenococcus oenitemperate bacteriophage phi 10MC,”FEMS Microbiol Lett, 171: 231-238 (1999).
Henikoff, “Undirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing,” Gene 28, 351 (1984).
Henrich et al., “Primary structure and functional analysis of the lysis genes ofLactobacillus gasseribacteriophage phi adh,”J Bacteriol, 177: 723-732 (1995).
Higuchi et al., “A general method of in vitri preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions,” Nucleic Acids Res. 16, 7351 (1988).
Hill et al., Identification of a lysin associated with a bacteriophage (A25) virulent for group A streptococci.J Bacteriol, 145: 696-703 (1981).
Inouye et al., “Bacteriophage T7 lysozyme is an n-acetylmuramyl-Lalanine amidase” Biol.Chem. 248, 7247 (1973).
Jain et al., “Use of lambda phage s and r gene products in an inducible lysis system for vibrio cholerae- and salmonella enterica serovar typhimurium-based DNA vaccine delivery systems,” Infect Immun, 68, 986 (2000).
Jerne, “Towards a network theory of the immune system,” Ann. Immunol. (Paris) 125c:373-389 (1974).
Jerne, N. K., et al., “Recurrent idiotopes and internal images,” EMBO 1:234 (1982).
Kaneko et al., “Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage phiPVL carrying Panton-Valentine leukocidin genes,”Gene, 215:57-67 (1998).
Kohler et al., “Continuous cultures of fused cells secreting antibody of predefined specifici

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