Inactivation of genes of the MEP pathway

Details Inactivation of genes of the MEP pathway Inactivation of genes of the MEP pathway

2011-01-25

2011-01-25

Ford, Vanessa L (Department: 1645)

Drug, bio-affecting and body treating compositions

Immunoglobulin, antiserum, antibody, or antibody fragment,...

Monoclonal antibody or fragment thereof

C424S130100, C424S001110, C424S093400, C435S069100

Reexamination Certificate

active

07875279

ABSTRACT:
The invention relates to cells and organisms as well as to methods for producing said cells and organisms, according to which intermediates of the mevalonate-independent pathway for isoprenoid biosynthesis (MEP pathway) are enriched by deleting or inactivating genes. The derivatives can also be enriched by using enzyme inhibitors. The enriched intermediates may be used as substrates in enzyme activity tests. The inventive cells and organisms and the enriched intermediates can further be used in the production of medicaments.

REFERENCES:
patent: WO 99/53071 (1999-10-01), None
patent: WO 99/58649 (1999-11-01), None
patent: WO 00/72022 (2000-11-01), None
Gustafson, C.E. et al. “Identification of theEscherichia colilytB Gene, Which Is Involved in Penicillin Tolerance and Control of the Stringent Response”Journal of Bacteriology, Feb. 1993, pp. 1203-1205, vol. 175, No. 4.
Bouvier, J. et al. “Nucleotide sequence of theIsp-dapBinterval inEscherichia coli” Nucleic Acids Research, Oct. 31, 1990, vol. 19, No. 1.
Cunningham, F. et al., “Evidence of a Role for LytB in the Nonmevalonate Pathway of Isoprenoid Biosynthesis”, pp. 5841-5848,Journal of Bacteriology, Oct. 2000, vol. 182, No. 20.
Altincicek B. et al., “Cutty Edge: Human gamma/delta T cells are Activated by Intermediates of the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway of Isoprenoid Biosynthesis”,Journal of Immunology, Bd. 1666, Nr. 6, Mar. 2001, pp. 3655-3658.
Belmant C. et al., “3-Formyl-1-Butyl pyrophosphate: A Novel Mycobacterial Metabolite-Activating Human gamma-delta T Cells”,The Journal of Biological Chemistry, Bd. 274, Nr. 45, Nov. 1999, pp. 23079-32084.
Jomaa, H. et al., “Vgamma9/Vdelta2 T Cell Activation Induced by Bacterial Low Molecular Mass Compounds Depends on the 1-Deoxy-D-Xylulose 5-Phosphate Pathway of Isoprenoid Biosynthesis”,FEMS Immunology and Medical Microbiology, Bd. 25, 1999, pp. 371-378.
Hintz M. et al., “Indentification (E)-4-Hydroxy-3-Methyl-But-2-Enyl Pyrophosphate as a Major Activator For Human gammadelta T Cells inEscherichia coli”, FEBS Letters, Elsevier Science Publishers, Amesterdam, NL, Bd. 509, Nr. 2, Dec. 2001, pp. 317-322.
Feurle J. et al., “Escherichia coliProduces Phosphoantigens Acticated Human gammadelta T Cells”,Journal of Biological Chemistry, Bd. Nr. 1, Jan. 4, 2002, pp. 148-154.
Seemann M. et al., “Isoprenoid Biosynthesis Via the Methylerthritol Phosphate Pathway: Accumulation of 2-C-Methyl-d-Erythritol 2,4-Cyclodiphosphate in a gcpE Deficient Mutant ofEscherichia coli”, Tetrahedron Letters, Elsevier Science Publishers, Amsterdam, NL, Bd. 43, Nr. 5, Jan. 28, 2002, pp. 775-778.

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