Inactivation of enzymes

Details Inactivation of enzymes Inactivation of enzymes

1982-05-14

1984-11-20

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Enzyme inactivation by chemical treatment

435 4, 435 19, 435 21, 435 26, C12N 999, C12Q 100, C12Q 144, C12Q 142, C12Q 132

Patent

active

044839227

ABSTRACT:
A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of peracetic acid. In the preferred embodiment the treated serum is subsequently neutralized and dialyzed. Preferably, the serums are used as standards for calibration or as a control in assay kits, e.g. RIA, and EIA or enzyme kits, for the determination of specific ligands and enzymes.

REFERENCES:
patent: 4279994 (1981-07-01), Huang
patent: 4311791 (1982-01-01), Bernstein
Schweizer et al., Chemcial Abstracts 78: 39986m, (1973).

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