In vivo site-specific incorporation of...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S252300, C435S252330, C435S320100, C435S015000, C435S069100, C536S023200

Reexamination Certificate

active

11255601

ABSTRACT:
Methods and compositions for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid having a N-acetylgalactosamine moiety into a protein; optionally, the N-acetylgalactosamine-containing unnatural amino acid can be further modified with additional sugars.

REFERENCES:
patent: 5369017 (1994-11-01), Wong et al.
patent: 6331418 (2001-12-01), Roth
patent: 6927042 (2005-08-01), Schultz et al.
patent: WO 02/085923 (2002-10-01), None
patent: WO 02/086075 (2002-10-01), None
patent: WO 03/031464 (2003-04-01), None
patent: WO 2004/035605 (2004-04-01), None
patent: WO 2004/035743 (2004-04-01), None
patent: WO 2004/058964 (2004-07-01), None
patent: WO 2004/094593 (2004-11-01), None
patent: WO 2005/007624 (2005-01-01), None
patent: WO 2005/007870 (2005-01-01), None
patent: WO 2005/019415 (2005-03-01), None
Expanding the Genetic Code. Wang et al. Angew. Chem.Int.Ed. 2005, vol. 44, pp. 34-66) which was published after the filing date of the instant application.
Arslan et al., (1997) “Structurally Modified Firefly Luciferase. Effects of Amnio Acid Substitution at Position 286.”Journal of the American Chemistry Society, 119(45): 10877-10887.
Bertozzi and Kiessling (2001) “Chemical Glycobiology.”Science, 291: 2357-2364.
Brick et al. (1989) “Structure of Tyrosyl-tRNA Sythetase Refined at 2-3 A Resolution: Interaction of the Enzyme with the Tyrosyl Adenylate Intermediate.”Journal of Molecular Biology, 208: 83-98.
CAO et al. (1995) “Stereoselective Phase Transfer Catalyzed Syntheses of Glycosyloxsuccinimides and their Transformation into Glycoprobes.”Tetrahedron, 51(24): 6679-6686.
Chin and Schultz (2002) “In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis.”ChemBioChem, 11: 1135-1137.
Chin et al. (2002) “Addition of a photocrosslinking amino acid to the genetic code ofEscherichia coli.” Proceedings of the National Academy of Sciences, USA, 99(17): 11020-11024.
Chin et al., (2003). “An Expanded Eukaryotic Genetic Coce.”Science, 301: 964-967.
Chin et al., (2002) “Addition of p-Azido-L-phenylalanine to the Genetic Code ofEscherichia coli,” Journal of the American Chemistry Society, 124: 9026-9027.
Davis and Flitsch (1991) “A Novel Method for the Specific Glycosylation of Proteins.”Tetrahedron Letters, 32(46): 6793-6796.
Davis (2002) “Synthesis of Glycoproteins.”Chem. Rev., 102: 579-601.
Dougherty (2000) “Unnatural Amino Acids as Probes of Protein Structure and Function.”Current Opinion in Chemical Biology, 4: 645-652.
Feng et al. (2003) “Expanding tRNA recognition of a tRNA synthetase by a single amino acid change,”Proceedings of the National Academy of Sciences, USA, 100(10): 5676-5681.
Forster et al. (2003) “Programming peptidomimetic syntheses by translating genetic codes designed de novo.”Proceedings of the National Academy of Sciences, USA, 100(11): 6353-6357.
Hang and Bertozzi (2001) “Chemoselective Approaches to Gylcoprotein Assembly.”Accounts of Chemical Research, 34: 727-736.
Hanish (2001) “O-glycosylation of the mucin type.”Biol Chem, 328: 143-149.
Koller et al.(2000) “Chemoenzymatic Synthesis of PSGL-1 Glycopeptides: Sulfation on Tyrosine Affects Glycosyltransferase-Catalyzed Synthesis of theO-Glycan.”Bioorganic and Medicinal Chemistry, 8:1017-1025.
Lamarre-Vincent and Hsieh-Wilson (2003) “Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation,”Journal of the American Chemistry Society, 125: 6612-6613.
Liu and Schultz (1999) “Progress toward the evolution of an organism with an expanded genetic code.”Proceedings of the National Academy of Sciences, USA 96, 4780-4785.
Macmillan et al. (2002) “Solid-Phase Synthesis of Thioether-Linked Glycopeptide Mimics for Application to Glycoprotein Semisynthesis.”Organic Letters, 4: 1467-1470.
Santoro et al. (2002) “An efficient system for the evolution of aminoacyl-tRNA synthetase specificity.”Nature Biotechnology, 20: 1044-1048.
Sarkar, et al., (1995) “Disaccharide uptake and priming in animal cells: Inhibition of sialyl Lewis X by acetylated Galβ1→GlcNAc β-O-naphthalenemethanol.”Proceedings of the National Academy of Sciences, USA, 92: 3323-3327.
Schultz and Kunz (1995) “Chemical and enzymatic synthesis of glycopeptides.”Interface between Chemistry and Biochemistry, 73: 201-228.
Sears and Wong (2001) “Toward Automated Synthesis of Oligosaccharides and Glycoproteins.”Science, 291: 2344-2350.
Shin et al. (1999) “Fmoc-Bases Synthesis of Peptide-αThioesters: Applications to the Total Chemical Synthesis of Glycoprotein by Native Chemical Ligation.”Journal of the American Chemistry Society, 121: 11684-11689.
Tolbert and Wong (2000) “Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes.”Journal of the American Chemistry Society, 122: 5421-5428.
Wacker et al. (2002) “N-Linked Glycosylation inCampylobacter jejuniand its Functional Transfer inE. coli,” Science, 298: 1790-1793.
Wang and Schultz (2002) “Expanding the Genetic Code.”Chem Comm, 1-11.
Wang and Schultz (2005) Expanding the Genetic Code, Angewandte Chemie 44: 34-66.
Wang et al. (2002) “Adding L-3-(2-Naphthyl)alanine to the Genetic Code ofE. coli,” Journal of the American Chemistry Society, 124(9): 1836-1837.
Wang et al. (2003) “Addition of the keto functional group to the genetic code ofEscherichia coli,” Proceedings of the National Academy of Sciences, USA, 100(1): 56-61.
Wang and Schultz (2001) “A general approach for the generation of orthogonal tRNAs.”Chemistry&Biology, 8: 883-890.
Wang et al. (2001) “Expanding he Genetic Code ofEscherichia coli,” Science, 292: 498-500.
Wells et al. (2001) “Glycosylation of Nucleocytoplasmic Proteins: Signal Transduction and O-GlcNAc.”Science, 291: 2376-2378.
Witte et al., (1998) “Solution- and Solid-Phase Synthesis of N-Protected Glycopeptide Esters of the Benzyl Type as Substrates for Subtilisin-Catalyzed Glycopeptide Couplings.”Journal of the American Chemistry Society. 120: 1979-1989.
Witte et al. (1997) “Enzymatic Glycoprotein Synthesis: Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation.”Journal of the American Chemistry Society, 119: 2114-2118.
Witte et al. (1998) “Solution-and Solid-Phase Synthesis of N-Protected Glycopeptide Esters of the Benzyl Type as Substrates for Subtilisin-Catalyzed Glycopeptide Couplings.”Journal of the American Chemistry Society, 120: 1979-1989.
Xie and Schultz (2005) “An Expanding Genetic Code.”Methods, 36: 227-238.
Zhang et al. (2004) “A New Strategy for the Synthesis of Glycoproteins.”Science, 303: 371-373.
Zhang et al. (2003) “A New Strategy for the Site-Specific Modification of Proteins in Vivo.”Biochemistry, 42: 6735-6746.
Zhang et al. (2002)“The Selective Incorporation of Alkenes into Proteins inEscherichia coli,” Angew. Chem. Int. Ed. Engl., 41(15): 2840-2842.
Rodriguez et al. (1998) “Aminooxy-, Hydrazide-, and Thiosemicarbazide-Functionalized Saccharides: Versatile Reagents for Glycoconjugate Synthesis,”J. Org. Chem., 63:7134-7135.

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