In vivo selection of RNA-binding peptides

Details In vivo selection of RNA-binding peptides In vivo selection of RNA-binding peptides

1995-05-17

1998-11-10

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352521, C12Q 168, C12N 120

Patent

active

058341846

ABSTRACT:
The invention provides methods and kits suitable for large-scale in vivo screening of polypeptides for specific binding affinity to a selected RNA recognition sequence. Polypeptide(s) are screened as fusion proteins with a polypeptide having antitermination activity. Polypeptides having binding activity are identified by their capacity to activate transcription of a reporter gene via binding of the polypeptide being screened to the selected RNA recognition sequence. The invention also provides methods of screening RNA molecules for binding to a selected polypeptide.

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