Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1993-05-06
1995-07-18
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 911, 435 9141, 435243, 4352523, 935 79, C12N 1509, C12N 1510
Patent
active
054340659
ABSTRACT:
A genetic system termed in vivo expression technology was devised that positively selects for microbial genes that are specifically induced when microbes infect their host. The method of this invention comprises complementing the growth of an auxotrophic or antibiotic sensitive microorganism by integrating an expression vector by way of homologous recombination into the auxotrophic or antibiotic sensitive microorganism's chromosome and inducing the expression of a synthetic operon which encodes transcripts, the expression of which are easily monitored both in vitro and in vivo.
REFERENCES:
Mahan, M. J. et al. 1993. Science vol. 259 pp. 686-688.
Barinaga, M. 1993. Science vol. 259 p. 595.
"Environmental Signals Controlling Expression of Virulence Determinants in Bacteria," by J. Mekalanos, J. Bacteriology 174, 1 (1992).
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"A Single Genetic Locus Encoded by Yersinia pseudotuberculosis Permits Invasion of Cultured Animal Cells by Escherichia coli K-12," by R. Isberg, et al., Nature, 317, 262-264 (1985).
"Identification of Plunt Induced Genes of the Bacterial Pathogen Xanthomonas campestris Pathovar compestris using a Promoter-Probe Plasmid," by A. E. Osborn, et al. EMBO J., 6, 23-28 (1987).
"Two trans-acting Regulatory Genes (vir and mod) Control Antigenic Modulation in Bordetella pertussis", by S. Knapp, et al., J. Bacteriol 170, 5059-5066 (1988).
"A Novel Suicide Vector and its use in Construction of Insertion Mutation: Osmoregulation of Outer Membrane Proteins and Virulence Determinants in Vibrio cholera Requires toxR," by V. Miller, et al. J. Bacteriol 170, 2575-2583 (1988).
Mahan Michael J.
Mekalanos John J.
Slauch James M.
Mosher Mary E.
Petersen Steven C.
President and Fellows of Harvard College
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