In vivo incorporation of alkynyl amino acids into proteins...

Details In vivo incorporation of alkynyl amino acids into proteins... In vivo incorporation of alkynyl amino acids into proteins...

2005-09-20

2010-11-02

Rao, Manjunath N (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S252100, C435S252300, C435S252800, C435S320100, C536S023200

Reexamination Certificate

active

07824893

ABSTRACT:
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such asE. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.

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