Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2003-01-16
2008-03-04
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100, C435S252300
Reexamination Certificate
active
07338800
ABSTRACT:
The present invention is directed to an in vivo method of transferring DNA from a donor cell to a recipient cell. In a specific embodiment, the method comprises a highly efficient process of bacterial mating. In a preferred embodiment, selection for a recombinant plasmid against a parent host plasmid and donor plasmid is based on recircularization of the host plasmid by its recombination with a gene of interest such that it is now no longer cleaved by a restriction enzyme expressed in the recipient cell.
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Elledge Stephen J.
Li Mamie Z.
Baylor College of Medicine
Fulbright & Jaworski LLP
Vogel Nancy
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