Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-03-02
1998-03-17
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 4351723, 435183, 4353201, 536 253, C12N 900, C12N 1566
Patent
active
057285519
ABSTRACT:
We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target DNA has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into DNA targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
REFERENCES:
patent: 5137829 (1992-08-01), Nag et al.
patent: 5212080 (1993-05-01), Nag et al.
patent: 5227288 (1993-07-01), Blattner
Kasai et al. "Efficient Large-scale Sequencing of the Escherichia coli Genome: Implementation of a transposon-and PCR-based Strategy for the Analysis of Ofrdered lambda Phage Clones", Nucleic Acids Research, vol. 20, No. 24, pp. 6509-6515, 1992.
Eichinger et al. "A Specific Terminal Structure is Required For Ty1 Transposition", Genes and Development, vol. 4, pp. 324-330, 1990.
Dower et al. "High Efficiency Transformation by High Voltage Electroporation", Nucleic Acids Research, vol. 16, No. 13, pp. 6127-6145, 1988.
Methods in Enzymology, vol. 152, pp. 560-562, 1987.
High efficiency transformation of E coli by high voltege Electroporation Dower, W.J. et al (1988) Nucleic Acid Research vol. 16 No. 13,6127-6145.
Methods in enzymology (1987) vol. 152 pp. 560-562 Academic Press.
Kasai, et al., "Efficient Large-Scale Sequencing of the Escherichia coli Genome: Implementation of a Transposon-and PCR-Based Strategy for the Analysis of Ordered .lambda.Phage Clones", Nucleic Acids Research, 20(24):6509-6515 (1992).
Phadnis, et al., "Tn5supF, a 264-Base-Pair Transposon Derived from Tn5 for Insertin Mutagenesis and Sequencing DNAs Cloned in Phage .lambda.", Proc. Natl. Acad. Sci. USA, 86:5908-5912 (1989).
Strathmann, et al., "Transposon-Facilitated DNA Sequencing", Proc. Natl. Acad. Sci. USA, 88:1247-1250 (1991).
Seifert, et al., "Shuttle Mutagensis: A Method of Transposon Mutagenesis for Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA", 83:735-739 (1986).
Ahmed, "A Vector for Sequencing Long (40-kb) DNA Fragments", Gene, 75:315-321 (1988).
Way, et al., "New TN10 Derivatives for Transposon Mutagenesis and for Construction of lacA Operon Fusions by Transposition", Gene, 32:369-379 (1984).
Kleckner, et al., "Uses of Transposons with Emphasis on TN10", Methods in Enzymology, 204:139-180 (1991).
Eichinger, et al., "The DNA Intermediate in Yeast Ty1 Element Transposition Copurifies with Virus-Like Particles: Cell-Free Ty1 Transposition," Cell, 54:955-966 (1988).
Brown, et al., "Correct Integration of Retroviral DNA in vitro", Cell, 49:347-356 (1987).
Eichinger, et al., "A Specific Terminal Structure is Required for Ty1 Transposition", Genes & Development, 4:324-330 (1990).
Boeke Jef D.
Braiterman Lelita T.
Devine Scott E.
Nelson Amy J.
Robinson Douglas W.
The Johns Hopkins University
LandOfFree
In vitro transposition of artificial transposons for DNA sequenc does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with In vitro transposition of artificial transposons for DNA sequenc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro transposition of artificial transposons for DNA sequenc will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-956628