Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-03-02
1997-10-14
Degen, Nancy
Chemistry: molecular biology and microbiology
Vector, per se
435 9141, 536 237, C12N 1565, C12N 1570, C12N 1511, C07H 2104
Patent
active
056771703
ABSTRACT:
We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
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Boeke Jef D.
Braiterman Lelita T.
Devine Scott E.
Degen Nancy
The Johns Hopkins University
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