In vitro transposition of article transposons

Details In vitro transposition of article transposons In vitro transposition of article transposons

1997-02-06

1999-10-19

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 913, 4353201, 536 237, C12N 1564, C12P 1934, C07H 2104

Patent

active

059687851

ABSTRACT:
We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

REFERENCES:
patent: 5677170 (1997-10-01), Devine et al.
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