Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se
Reexamination Certificate
2003-06-03
2008-12-30
Para, Annette H (Department: 1661)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
C504S117000, C047S05810R
Reexamination Certificate
active
07470832
ABSTRACT:
A highly efficient in-vitro system of micropropagation of rose scented Geranium,Pelargonium graveolensL. Herit by a direct regeneration method to produce a large number of viable true to the type plants maintaining the genotype of an elite mother plant is provided. The process involves inoculating nodal explants on shoot regeneration and multiplication medium, transferring the multiple shots for further growth on medium for shoot growth, further transferring the shoot with sufficient growth to medium for rooting. The present invention also provides a process for the primary and secondary hardening of the in vitro generated plants with the efficient root regeneration system, which is hardened to give about 95% survival in the field conditions. The multiplication ratio achieved by the process is of the order of 1:12-1:20, resulting in significantly low cost of production in relatively lesser time.
REFERENCES:
Gupta et al. Micropropagation of Elite Cultivars of Rose-scented Geranium (Pelargonium graveolensL' Herit.) for industrial Production of Propagules 2002 Indian Journal of Biotechnology pp. 286-291.
Smith Roberta H. Plant Tissue Culture. 2000. Techniques and experiments second edition. Academic Press. 43-58, 70-81.
Arockiasamy D. I. et al. Plant regeneration from node and Internode Explants of Solanum triblobatum I. 2002. Plant Tissue Cult. 12(2): 93-97.
Arockiasamy et al. Plant Regeneration from Node and Internode Explants of Solanum Trilobatum L. Plant Tissue Cult. 12(2)2002.
Saxena et al. Composition of the essential oil of a New Isomenthone-Rich variant of geranium Obtained from Geraniol-Rich Cultivar ofPelargoniumSpecies. Journal of essential Oil Research Mar./Apr. 2004. Abstract.
Perez et al. Micropropagation of fazinus angustifolia from mature and juvenile plant material. Plant Cell, Tissue and Organ Culture 37: 297-302 1994.
Kumar Anil Kush
Patnaik Debasis
Lackenbach & Siegel LLP
Nissen J. Harold
Para Annette H
Reliance Life Sciences Pvt. Ltd.
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