In vitro propagation of grape

Plant husbandry – Process

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A01G 100

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045327335

ABSTRACT:
A process for controlling the development of somatic embryos for either a self-replicative or a vine development mode. Somatic embryogenesis is initiated by placing the embryo in a medium conducive for self-replication. When the embryos have grown to a first stage, they are transferred to a medium with cytokinin activity. They continue to grow until they have grown to a second stage at which time they are transferred to a medium without cytokinin activity until they have grown sufficiently and can be transferred to a non-liquid growth medium.

REFERENCES:
patent: 4241536 (1980-12-01), Saint-Firmin
patent: 4301619 (1981-11-01), Janick et al.
M. Barlass, K. G. M. Skeen, "In Vitro Propagation of Grapevine (Vitis vinra L.) from Fragmented Shoot Apices" (1748) Vitis 17:335-340.
Krul et al., "Formation of Adventitious Embryo in Callus Cultures of `Seyval`", J. Amer. Soc. Hort. Sci. 102:360-363.
M. G. Mullins et al., "Somatic Embryos and Plantlets from an Ancient Clone of the Grapevine (cv. Cabernet-Sauvignon) by apomixis in vitro," (1976), J. Expt. Bot. 27:1022-1030.
T. Murashige, "Plant Propagation Through Tissue Cultures", (1974), Annu. Rev. Plant Physiol. 25:135-166.
F. C. Steward et al., "Growth and Organized Development of Cultured Cells; II. Organization in Cultures Grown from Freely Suspended Cells," (1958), Amer. J. Bot. 45:705-708.
W. R. Krul, "In Vitro Propagation of Grape", (1980); Journal Art. No. 1932 Rhode Island Agricultural Experimental Station.
Murashige et al., "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures", (1962) Physiologia Plantarum, vol. 15:473-497.
Judith Myerson, "Adventitious Embryo-Genesis and Plantlet Development in Cultures of Vitis vinifera L. `Seyval`", Thesis, Univ. of Rhode Island, 1981, 249 pages.

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