Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1993-12-23
1996-04-16
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
4351723, 4353171, C12N 120, C12N 700, C12N 1509
Patent
active
055081878
ABSTRACT:
A bacterial preparation capable of packaging phage .lambda. DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [.lambda. c/857 Sam7 .DELTA.(cos-Nu1-A)::Kn.sup.r ]/.lambda. pTER and is capable of a packaging efficiency of at least 1.times.10.sup.8 pfu/.mu.g wild type .lambda. DNA. The present invention is also a method of creating a phage .lambda. DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage .lambda. and lyophilizing said extract.
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Frackman Susan G.
Franciskovich Phillip P.
Jolly James F.
Luhm Robert A.
Riedl William A.
Carter Philip W.
Fleisher Mindy B.
Pharmacia P-L Biochemicals Inc.
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