In vitro peptide or protein expression library

Details In vitro peptide or protein expression library In vitro peptide or protein expression library

1998-02-18

2008-08-26

Wessendorf, T. D. (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S006120, C435S005000, C435S091500, C435S091500

Reexamination Certificate

active

07416847

ABSTRACT:
The present invention relates to methods of producing peptide or protein expression libraries. In such a library, a population of nucleotide sequences is expressed. The resulting peptides or proteins encoded by those nucleotide sequences are then screened to identify those peptides or proteins having a desired property such as the ability to bind a selected ligand. In the construction and screening of such libraries, it is desired to ensure that the nucleotide encoding a particular protein remains connected in some way with that protein so that once a desired protein having a selected property has been isolated, its encoding nucleotide sequence may be specifically recovered for subsequent manipulation for example using PCR or sequencing. The present invention makes use of a property not previously described in constructing an expression library namely the use of proteins which covalently bind DNA. DNA is expressed in such a way that the protein so expressed binds to its own encoding DNA therefore allowing the DNA to be associated only with its encoding protein.

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FitzGerald, “In vitro display technologies—new tools for drug discovery”, DDT 5(6):253-258 (2000).

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