In Vitro mutagenesis, phenotyping, and gene mapping

Details In Vitro mutagenesis, phenotyping, and gene mapping In Vitro mutagenesis, phenotyping, and gene mapping

2007-04-24

2007-04-24

Qian, Celine (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S468000, C435S441000, C435S443000, C435S444000, C435S446000, C435S447000, C435S448000, C435S440000, C435S325000, C435S366000, C435S419000

Reexamination Certificate

active

10428977

ABSTRACT:
Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; inducing site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.

REFERENCES:
patent: 5658772 (1997-08-01), Odell et al.
patent: 5780296 (1998-07-01), Holloman et al.
patent: 5789215 (1998-08-01), Berns et al.
patent: 5885836 (1999-03-01), Wahl et al.
patent: 5965415 (1999-10-01), Radman et al.
patent: 6060296 (2000-05-01), Hoekstra
patent: 6077667 (2000-06-01), Bradley et al.
patent: 6139833 (2000-10-01), Burgess et al.
patent: 6159688 (2000-12-01), Borchert et al.
patent: 6159690 (2000-12-01), Borrebaeck et al.
patent: 6207371 (2001-03-01), Zambrowicz et al.
patent: 6232112 (2001-05-01), Catcheside
patent: 6238884 (2001-05-01), Short et al.
patent: 6255113 (2001-07-01), Zarling et al.
patent: 6277588 (2001-08-01), Freeman et al.
patent: 6277608 (2001-08-01), Hartley et al.
patent: 6284541 (2001-09-01), Auer et al.
patent: 6294346 (2001-09-01), Weiss et al.
patent: 6303327 (2001-10-01), Von Melchner et al.
patent: 6319692 (2001-11-01), Kadota et al.
patent: 6344356 (2002-02-01), Stemmer
patent: 6352859 (2002-03-01), delCardayre et al.
patent: 2001/0008026 (2001-07-01), Schneider et al.
patent: 2002/0123058 (2002-09-01), Threadgill et al.
patent: 2002/0127715 (2002-09-01), Benvenisty et al.
patent: 2003/0027335 (2003-02-01), Ruley et al.
patent: WO99/25851 (1999-05-01), None
patent: WO99/67361 (1999-12-01), None
patent: WO00/04190 (2000-01-01), None
patent: WO00/54574 (2000-09-01), None
patent: WO01/23545 (2001-04-01), None
Chen et al. Genotype-based screen for ENU-induced mutations in mouse embryonic stem cells. Nat Genet. vol. 24, No. 3, pp. 314-317, Mar. 2000.
Bronstein et al. Modulation of ethylnitrosourea-induced toxicity and mutagenicity in human cells by O6-benzylguanine. Cancer Res. vol. 52, No. 14, pp. 3851-3856, Jul. 1992.
Milstone et al. Simultaneous Cre catalyzed recombination of two alleles to restore neomycin sensitivity and facilitate homozygous mutations. Nucleic Acids Res. vol. 27, No. 15, p. e10 (pp. i/iii to iii/iii), Aug. 1999.
Lefebvre et al. Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity. Nat Genet. vol. 27, No. 3, pp. 257-258, Mar. 2001.
Weiss et al. In vitro differentiation of murine embryonic stem cells. New approaches to old problems. J Clin Invest. vol. 97, No. 3, pp. 591-595, Feb. 1996.
Liu et al. Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells. Nat Genet. vol. 30, No. 1, pp. 66-72. Epub Dec. 10, 2001.
Beard et al. Stress responses to DNA damaging agents in the human colon carcinoma cell line, RKO. Mutation Research, vol. 371, pp. 1-13, 1996.
Ramirez-Solis et al. Chromosome engineering in mice. Nature, vol. 378, pges 720-724, 1995
Zheng et al. Engineering a mouse balancer chromosome. Nature Genetics, vol. 22, pp. 375-378, 1999.
Koike et al. Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination. EMBO Reports, vol. 3, No. 5, pp. 433-437, 2002.
Adams et al., Induced mitotic recombination: a switch in time,Nature Genetics30:6-7 (Jan. 2002).
Araki et al., Targeted integration of DNA using mutant lox sites in embryonic stem cells,Nucleic Acids Research25(4):868-872 (1997).
Araki et al., Exchangeable gene trap using the Cre/mutated lox system,Cell. Mol. Biol.45(5):737-750 (Jul. 1999).
Albert et al., Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome,Plant J. 7(4):649-659 (Apr. 1995) (ABSTRACT).
Abbate et al., Bifunctional protein conferring enhanced green fluorescence and puromycin resistance,Biotechniques31(2):336-340 (Aug. 2001) (ABSTRACT).
Beckers et al., Large-scale mutational analysis for the annotation of the mouse genome,Proteomics and Genomics17-23 (published online Nov. 29, 2001).
Bentley et al., DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability,J. Cell Sci.115(7):1551-1561, (Apr. 2002).
Blake, et al., The Mouse Genome Database (MGD): the model organism database for the laboratory mouse,Nucleic Acids Research30(1):113-115 (2002).
Buchholz et al., Improved properties of FLP recombinase evolved by cycling mutagenesis,Nat Biotechnol.16)7):657-662 (Jul. 1998) (ABSTRACT).
Chen et al., CREating breakthroughs,Nature Biotechnology19:921-922 (Oct. 2001).
Chen, et al., A new positive
egative selectable marker, puDeltatk, for use in embryonic stem cells,Genesis28(1):31-35 (Sep. 2000) (ABSTRACT).
Cohen-Tannoudji et al., I-Scel-Induced Gene Replacement at a Natural Locus in Embryonic Stem Cells,Molecular and Cellular Biology18(3):1444-1448 (Mar. 1998).
Derossi et al., Trojan peptides: the penetratin system for intracellular delivery,Cell Biology8:84-87 (Feb. 1998).
De Berardino, Animal Cloning—the route to new genomics in agriculture and medicine,Differentiation68:67-83 (2001).
Duffy et al., Identifying loci required for follicular patterning using directed mosiacs,Development125:2263-2271 (1998).
Esposito et al., The integrase family of tyrosine recombinases: evolution of a conserved active site domain,Nucleic Acids Research25(18):3605-3614 (1997).
Farley et al., Widespread Recombinase Expression Using FLPeR (Flipper) Mice,Genesis28:106-110 (2000).
Flaherty, Generation, Identification, and Recovery of Mouse Mutations,METHODS: A Companion to Methods in Enzymology14:107-118 (1998).
Grainge et al.,The integrase family of recombinase: organization and function of the active site,Mol Microbiol.33(3):449-456 (Aug. 1999) (ABSTRACT).
Groth et al., A phage integrase directs efficient site-specific integration in human cells,PNAS97(11):5995-6000 (May 23, 2000).
Halbert et al., Adeno-associated Virus Vectors Transduce Primary Cells Much Less Efficiently then Immortalized Cells,J. of Virology69(3):1473-1479 (Mar. 1995).
Hanin et al., Gene targeting in Arabidopsis,The Plant Journal28(6):671-677 (2001).
Hérault et al., Engineering chromosomes in mice through targeted meiotic recombination (TAMERE),Nature Genetics20:381-384 (Dec. 1998).
Hrabé de Angelis et al., Genome-wide large-scale production of mutant mice by ENU mutagenesis,Nature Genetics25:444-447 (Aug. 2000).
Isaacs et al., Identification of two new pmp22 mouse mutants using large-scale mutagenesis and a novel rapid mapping strategy,Human Molecular Genetics9(12):1865-1871 (2000).
Jo et al., Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase,Nature Biotechnology19:929-933 (Oct. 2001).
Kanbashi et al., Frameshifts, base substitutions and minute deletions constitute X-ray-induced mutations in the endogenous tonB gene ofEscherichia coliK12,Mutation Research385:259-267 (1997).
Kanegae et al., Efficient gene activiation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase,Nucleic Acids Research23(19):3816-3821 (Oct. 1995) (ABSTRACT).
Kolot et al., Site-specific recombination in mammalian cells expression the Int recombinase of bacteriophage HK022,Mol. Biol. Rep.26(3):207-213 (Aug. 1999)(ABSTRACT).
Kurtzman et al., Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins,Biotechnology12:361-370 (2001).
Lebel et al., A deletion within the murine Werner syndrome helicase induces sensitivity to inhibitors of topoisomerase and lose of cellular proliferative capacity,Proc. Natl. Acad. Sci. USA95:13097-13102 (Oct. 1998).
Lee et al., Role of nucleotide sequences of IoxP spacer region in Cre-mediated recombination,Gene216:55-65 (1998).
Lindgren et al., Cell-penetrating peptides,TiPS21:99-103 (Mar. 2000).
Maeser et al. The Gin recombinase of pha

Affiliated with

Also associated with

Rating

In Vitro mutagenesis, phenotyping, and gene mapping does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with In Vitro mutagenesis, phenotyping, and gene mapping, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In Vitro mutagenesis, phenotyping, and gene mapping will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3788508