In-vitro methods for identifying compositions which are agonists

Details In-vitro methods for identifying compositions which are agonists In-vitro methods for identifying compositions which are agonists

1986-04-17

1989-08-22

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C12Q 102

Patent

active

048595853

ABSTRACT:
The present invention provides two general protocols by which a substance may be identified and characterized as an estrogen agonist and/or an estrogen antagonist. The protocols are in-vitro methods which utilize estrogen dependent cells in culture and a medium comprising an inhibitor endogenous to the sera of adult humans which is able to prevent the proliferation of cultured cells in-vitro. The methodology is rapid, reproducible, accurate and provides the major advantage of being able to screen large numbers of substances for their primary or secondary side effects in a large variety of medical and environmental applications.

REFERENCES:
Coezy et al, Cancer Research, 42, 317-323, 1982.
Reddel et al, Cancer Research, 43, 4618-4624, 1983.
Soto et al, Biochem. Biophys. Res. Communic., 122, 1097-1103, 1984.
Biotechnology, Fisher Scientific, 1983, p. 97.
Sonneschein et al, J. Cell Biol., 99, 3392, 1984.
Sonneschein et al, Biol. Abstr., 80, Abstr. No. 69567, 1985.
Stack & Gorski, Endocrinology, 115: 1141-1150 (1984).
Sonnenschein & Pierce, Cancer Research, 41: 4742-4743 (1981).
Laugier et al., Proc. Natl. Acad. Sci. USA, 80: 1621-1625 (1983).
Soto & Sonneschein, J. Cell Biol., 97: 393a (1983).
Soto & Sonnenschein, Proc. Am. Assoc. Can. Res., 25: 213 (1984).
Schatz et al., Endocrinology, 115: 501-506 (1984).
Paz et al., J. Cell Biol., 99: 339a, No. 1256 (1984).
Sonnenschein & Soto, Proc. AACR, 26: 198, No. 783 (1985).
Soto et al., Proc. AACR, 26: 198, No. 784 (1985).
Soto & Sonnenschein, Endo. Soc., 67th Annual Mtg., No. 1152 (1985).
Sonnenschein & Soto, Endo. Soc., 67th Annual Mtg., No. 663 (1985).
Sonnenschein et al., Life Sciences, 37: 387-394 (1985).
Soto & Sonnenschein, J. Steroid. Biochem., 23: 87-94 (1985).
Schatz et al., J. Cell. Physiol., 124: 386-390 (1985).
Papendorp et al., J. Cell. Physiol., 125: 591-595 (1985).

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