In vitro method to generate dendritic Langerhans type cells

Details In vitro method to generate dendritic Langerhans type cells In vitro method to generate dendritic Langerhans type cells

2001-03-05

2009-12-29

Ewoldt, G. R (Department: 1644)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S383000

Reexamination Certificate

active

07638329

ABSTRACT:
Described is an in vitro method for developing dendritic Langerhans type cells, for use in immunotherapy against cancer or infectious diseases and enhancement of graft survival in mammals. The method includes the steps of: culturing cells selected from peripheral blood monocytes and bone marrow cells in a medium containing platelets obtained from the same species or phylogenetically close species; and incubating the culture at 30 to 40° C. for a period sufficient to enable formation of mature dendritic Langerhans type cells.

REFERENCES:
patent: 6828157 (2004-12-01), Pankowsky
Steinman, R. Dendritic Cells, in Fundamental Immunology. Paul, W., ed. 1999;p. 523.
Williams et al. Isolation and Function of Human DCs, in International Review Cytology. 1994; vol. 153, pp. 54-57.
Caux et al. J. Exp. Med. 1996; vol. 184, pp. 695-706.
Semple, J.W., et al. Blood, Nov. 15, 1991; 78(10):2619-2625.
Bone Marrow, www.wikepedia.org.
Brand, C.U., et al. Arch. Dermatol. Res. 1999;291:65-72.
Romani. N., et al. AMPIS. 2003;111:725-740.
PathologyOutlines internet site, outline/chapter entitled “CD Markers”, entry: CD83, last visited Jul. 22, 2003.
Schoppet M et al., 2003, “CD83+ dendritic cells in inflammatory infiltrates of Churg-Strauss myocarditis”,Arch Pathol Lab Med. 127:98-101.
Geissmann F et al., 2001, “Differentiation of Langerhans cells in Langerhans cell histiocytosis”,Blood97:1241-1248.
Satthaporn S et al., 2001, “Dendritic cells (I): Biological functions”,J R Coll Surg Edinb. 46: 9-19.
Hirano A et al., 2000, “Graft hyporeactivity induced by donor-derived dendritic cell progenitors”,Transplant Proc. 32:260-264.
Chakraborty NG et al., 1999, “Emergence of regulatory CD4+ T cell response to repetitive stimulation with antigen-presenting cells in vitro: implications in designing antigen-presenting cell-based tumor vaccines”,J. Immunol. 162:5576-5583.
Dhodapkar MV et al., 1999, “Rapid generation of broad T-cell immunity in humans after a single injection of mature dendritic cells”,J. Clin. Invest. 104:173-180.
Banchereau J et al., 1998, “Dendritic cells and the control of immunity”,Nature392(6673):245-252.
Khanna A et al., 1998, “Donor bone marrow potentiates the effect of tacrolimus on nonvascularized heart allograft survival: association with microchimerism and growth of donor dendritic cell progenitors from recipient bone marrow”,Transplantation65:479-485.
Palucka KA et al., 1998, “Dendritic cells as the terminal stage of monocyte differentiation”,J. Immunol. 160:4587-4595.
Hsu FJ et al., 1996, “Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells”,Nature Medicine2:52-58.
Young JW et al., 1996, “Dendritic cells as adjuvants for class I major histocompatibility complex-restricted antitumor immunity”,J. Exp. Med. 183:7-11.
Caux C et al., 1992, “GM-CSF and TNF-alpha cooperate in the generation of dendritic Langerhan cells”,Nature360:258-261.
Steinman RM et al., 1974, “Identification of a novel cell type in peripheral lymphoid organs of mice. 3. Functional properties in vivo”,J. Exp. Med. 139:1431-1445; and.
Steinman et al., 1973, “Identification of a novel cell type in peripheral lymphoid organs of mice. I Morphology, quantitation, tissue distribution”J. Exp. Med. 137:1142-1162.

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