Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Reexamination Certificate
2011-08-16
2011-08-16
Nguyen, Bao Thuy L (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
C436S514000, C436S524000, C436S525000, C436S810000, C435S287100, C435S287700, C422S425000, C422S430000
Reexamination Certificate
active
07998754
ABSTRACT:
The invention concerns a diagnostic kit for simultaneous assay of antibiotics of different classes. The kit contains a single reaction mixture containing at least one first labelled receptor, specific of recognition of β-lactams, a second labelled receptor, specifically and competitively identifying a tetracycline and a biotinylated nucleic acid fragment, an antisulfamide antibody and a labelled sulfamide analogue; and a recovery system in the form of a solid support comprising a nitrocellulose membrane whereon are fixed in three separate testing zones, respectively an antibiotic with β-lactam nucleus, an avidin and an antibody capable of specifically identifying the antisulfamide antibody, so that the labelling intensity detected on the recovery system at the three testing zones results independently from a competitive recognition of each antibiotic by its labelled receptor.
REFERENCES:
patent: 5434053 (1995-07-01), Piasio
patent: 6319466 (2001-11-01), Markovsky et al.
patent: 6475805 (2002-11-01), Charm et al.
patent: 6524804 (2003-02-01), Degelaen et al.
patent: 2005/0130152 (2005-06-01), Granier et al.
patent: 0 593 112 (1994-04-01), None
patent: WO 99/67416 (1999-12-01), None
patent: WO 03/048770 (2003-06-01), None
Joris et al FEMS Microbiology Letters. vol. 70, No. 1. Jun. 15, 1990. pp. 107-113.
Rowe, C. et al. (1999) “An array immunosensor for simultaneous detection of clinical analytes” Anal. Chem. 71:433-439.
Golemi-Kotra, D. et al. (2003) “Resistance to B-lactam antibiotics and its mediation by the sensor domain of the transmembrane blar signaling pathway in staphylococcus aureus” The Journal of Biological Chemistry, 278:18419-18425.
Verheijen, R. et al. (1998) “Development of a one step strip test for the detection of sulfadimidine residues” Analyst 123:2437-2441.
Hisao Oka, Chemical Analysis for Antibiotics Used in Agriculture, by AOAC International, 1995, ISBN 0-935584-57-9.
Kachab et al., “The development of an enzyme-linked immunosorbent assay (ELISA) for cephalexin.” Journal of Immunology Methods, J Immunol Methods, 147(1):33-41, (1992).
Developing Immunochromatographic Test Strips: A Short Guide, Millipore, Lit. No. TB500 Printed in USA Nov. 1996, 96-204 SAME AS 01, © 2002, 2006.
Frens, G., “Controlled nucleation for the regulation of the particle size in mondisperse gold suspension” Nature (London), Phys. Sci., 241:20-22 (1973).
Dixon-Holland et al. “Competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue” J. Assoc. Off. Anal. Chem. 71:(6), pp. 1137-1140 (1988).
Knobbe Martens Olson & Bear LLP
Nguyen Bao Thuy L
Unisensor S.A.
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