In vitro method for the diagnosis of cardiovascular...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S374000

Reexamination Certificate

active

07919315

ABSTRACT:
The present invention relates to an in vitro method for the analysis of a sample from a mammal in connection with cardiovascular diseases, wherein the method comprises the following steps: a) isolating of bone marrow-precursor cells (BMPs) and/or blood-derived circulating precursor cells (BDPs) by means of cell specific surface markers, and b) detecting the cardiovascular functionality of the isolated BMPs and/or BDPs by means of a suitable migration assay. The method according to the invention can be employed as a kit in the context of the diagnosis and/or the prognosis of cardiovascular diseases, for the monitoring of their therapies and/or for a stratification for a prospective cell therapy with stem- and/or precursor cells in order to increase the perfusion of ischemic tissue or for a regeneration of tissue losses (e.g. heart insufficiency), respectively. In a further aspect, the present invention then relates to an in vitro method for isolating specific bone marrow-precursor cells by means of a suitable migration assay. According to the invention, these BMPs and/or BDPs can be used for the treatment of cardiovascular diseases, selected from the group consisting of stable coronary heart disease, acute coronary syndrome, acute myocardial infarction, chronic ischemic cardiomyopathy (ICMP), dilatative cardiomyopathy (DCM) or other causes for a heart insufficiency.

REFERENCES:
Vasa, M. et al., Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease,Circulation Research89(1): E1-E7, 2001.
Yamaguchi, J.-I. et al., Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization,Circulation107(9) 1322-1328, 2003.
Assmus, B. et al., Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction (TOPCARE-AMI),Circulation106(24) 3009-3017, 2002.
Kim, C.H. et al., In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: stromal cell-derived Factor-1, steel factor, and the bone marrow environment, Blood 91(1), 100-110, 1998.

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