In vitro method for stimulating cell growth by culturing cells i

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352402, 4352403, 43524031, C12N 500

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active

054686350

ABSTRACT:
The invention relates to an agent for promoting growth in animal cells. The agent contains a serum-free medium and lysozyme. The agent can optionally contain cytokines. The invention further relates to an in vitro method of stimulating the growth of T lymphoblastoid cells and peripheral blood lymphocytes, which comprises the step of culturing the T lymphoblastoid cells or peripheral blood lymphocytes in a serum-free culture medium comprising human lysozyme, as a growth stimulating agent, at a concentration of about 4-100 mg/liter.

REFERENCES:
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Sigma Chemical Co. 1981 Catalog p. 410.
Ruth E. Gordon, D. A. Barnett, J. E. Handorhan and C. H. Pang, Nocardia coelica, N. autotrophica, and the Nocardin Strain, Int. J. of Systematic Bacteriology 24:54-63, 1974.
ATCC catalogue of Cell Lines and Hybridomas, 16th edt. 1988, ATCC, Rockville, Md. pp. 3-5.
N. A. Dobrotina et al. [Lysozyme as a modulator], Vopr Med Khim 33(4):66-9, 1987.
J. R. Yanelli J. Imm. Meth. 139:1-6 1991.
Bjare et al., Pharmac. Ther., vol. 53, No. 3, pp. 355-374 (1992).
Jakoby et al., "Methods in Enzymology", vol. LVIII, published in 1979 by Academic Press, Inc. pp. 44-109.
Katsuta, H., "Kutritional Requirements of Cultured Cells", published in 1978 by University Park Press (Md.) pp. 1-21.
Barnes et al, Analytical Biochemistry, vol. 102, No. 2, pp. 255-269 (1980).
Oleinik et al, Immunologiya, O(3), pp. 78-81 (1982) (see biosis abstract) (translation attached).

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