In vitro method for producing infective bacterial spores and spo

Details In vitro method for producing infective bacterial spores and spo In vitro method for producing infective bacterial spores and spo

1986-03-24

1989-04-25

Weimar, Elizabeth C.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Conjugate or complex

424 95, 424 93, 435242, 435832, 435176, A01N 6300, C12N 300, C12N 1102, C12R 300

Patent

active

048246719

ABSTRACT:
Disclosed are insecticidal compositions for controlling Scarabaeide comprising an effective amount of sporangium-free spores of pathogens that cause milky disease in said Scarabaeide. Also disclosed is a method for producing infective milky disease bacillus spores in vitro in liquid media containing starch, trechalose, yeast extract, K.sub.2 HPO.sub.4, and CaCO.sub.3 during the vegetative growth stage and MnSO.sub.4 and optionally an ion-exchange resin as sporulation adjuvants.

REFERENCES:
patent: 3503851 (1970-03-01), Srinivasan
patent: 3790665 (1974-02-01), Glass et al.
patent: 3950225 (1976-04-01), Skole et al.
patent: 4626508 (1986-12-01), Steinkraus
patent: 4661351 (1987-04-01), Gago et al.
Costilow, R. N. et al., Physiological Studies of an Oligosporogenous Strain of Bacillus Popolliae; Appl. Microbiol., vol. 22, No. 6, (1971), pp. 1076-1084.
Sharpe, E. S. et al.; Characteristics of a New Strain of Bacillus Popilliae Sporogenic in Vitro; Appl. Microbiol. vol. 19, No. 4, (1970); pp. 681-688.
St. Julian, G. et al.; Preparation and Characterization of Intact and Free Spores of Bacillus Popilliae Dutky; Can. J. Microbiol. vol. 13 (1967); pp. 279-285.
Haynes, W. C. et al; Sporulation of Bacillus Popilliae in Liquid Medium as Affected by Kind of Carbon and Method of Sterilization; Can. J. Microbiol., vol. 18 (1972); pp. 515-518.

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