In vitro method for eye and skin irritation testing

Details In vitro method for eye and skin irritation testing In vitro method for eye and skin irritation testing

1997-06-18

2000-02-01

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436 63, C12Q 132

Patent

active

060201487

ABSTRACT:
A technique for in vitro testing of ocular and dermal irritants is disclosed. The process involves the topical application of liquid, solid granular or gel-like materials (e.g. cosmetics) to a cell culture and then evaluating the cytotoxicity of the material. Cell cultures of human skin without a stratum corneum and having a histologic similarity to the eye are used. Irritation is evaluated by measuring cell viability using MTT assay (based on the reduction of a tetrazolium dye by functional mitochondria), or release of LDH or PGE.sub.2. A unique method of applying test materials which are not water soluble is described.

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