In vitro method for concerted integration of donor DNA molecules

Details In vitro method for concerted integration of donor DNA molecules In vitro method for concerted integration of donor DNA molecules

1996-06-27

1998-09-22

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 6, 4351723, C12P 1934, C12Q 168, C12N 1510

Patent

active

058112707

ABSTRACT:
A method of analysis of concerted integration in which viral integrase enzyme is first incubated with donor DNA molecules followed by incubation with target DNA molecules. The donor DNA molecule having at least one unique restriction site for analysis of concerted integration product.

REFERENCES:
Fitzgerald et al. (1992) "Concerted Integration of Viral DNA Termini by Purified Avian Myeloblastosis Virus Integrase" J. Virol. 66:6257-6263.
Craigie et al. (1987) "Transposition of Mu DNA: Joining of Mu to Target DNA can be uncoupled from Cleavage at the end of Mu" Cell 51:493-501.

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