Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Multi-enzyme system
Reexamination Certificate
2002-11-01
2008-09-23
Redding, David A (Department: 3723)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Multi-enzyme system
C435S176000, C435S179000, C435S181000, C435S283100, C435S289100
Reexamination Certificate
active
07427497
ABSTRACT:
Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
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Clark Douglas S.
Dordick Jonathan S.
Kim Jung-bae
Sherman David H.
Srinivasan Aravind
Darpa
Hamilton Brook Smith & Reynolds P.C.
Redding David A
Rensselaer Polytechnic Institute
The Regents of the University of California
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